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The second case, Horse D, was treated by intravenous injections of methylene blue first, followed subsequently by injections of perchloride of mercury.

The third case. Horse E, was treated by injections of the mixture of methylene blue and perchloride of mercury. In reviewing the work on these three cases, it is well worth placing the charts side by side, so as to compare the effects of treatment on the temperature, and as to the presence of trypanosomes in the blood. To confirm the effects of the perchloride of mercury treatment in Horse C, two other horses, viz., F and G, were treated in the same way and these will be described later on.

Horse Cinoculated on the 14th February, 1908, with 2 c.c. blood from Calf C suffering from trypanosomiasis. On the 25th, trypanosomes were found in the blood and treatment was begun. 10 c.c. of perchloride of mercury solution (1 in 1.000) were injected intravenously every day for 10 days up to 5th March. A marked drop in the temperature occurred on the 27th February and again a sudden great rise to 106.2° F. on the 1st March, but examination of the blood showed no trypanosomes. On the 2nd March the temperature had again fallen to-normal and the blood again showed no trypanosomes, and, in order to verify this fact, c.c of blood from the horse were on that day inoculated subcutaneously into Dog J. The day after the last injection of perchloride of mercury the blood again showed no trypanosomes. The temperature of this horse now practically ran normal for one month and 17 days from 2nd March to 19th April, during which period try panosomes were not found in his blood. On the 24th March 22 days had passed since Dog J had been inoculated with 8 c.c. of blood, and, as this was well over the maximum period of incubation in dogs, namely, 12 days (see page 8), a series of daily injections varying in amount from 8 to 10 c.c. of blood into Dog J were begun with a view to rendering Dog J immune. At this time the blood of Horse C was considered to be free from trypanosomes as (1) Dog J had not developed the disease; (2) no trypanosomes could be found in the blood; and (3) the horse's tem- perature was running a normal course. Dog J was injected with blood every day for 10 days, so that by April 2nd the total quantity of blood inoculated was 94 c.c.

This will be referred to later under Section III. (see page 65).

On April 20th a slight rise in temperature occurred with trypanosomes in the blood, and a further series of intravenous injections-10 c.c. of perchloride of mercury solution (1 in 1,000) were made daily for five days, after which the temperature again ran normal, and the blood showed no trypanosomes.

On 5th May daily subcutaneous injections of blood into Dog F were begun, so that by the 5th day the dog had received 40 c.c. of blood. On the 10th May 8 c.c. blood was injected into Dog R.

On the 10th and 11th May, each day 8 c.c. of blood were injected into Monkey A, so that the monkey received a total quantity of 16 c.c. On 13th May daily injec- tions of blood were made into Horse H., so that by the 17th May Horse H. had received 81 c.c. blood.

These cases into which blood was injected will be referred to later on, under. Section III.

From this period-after these injections of blood were made into other animals--the horse's temperature ran normal, and no trypanosomes could be found in the blood up to the time of death, but the animal fell off in condition, and succumbed on the 12th July-a period of 4 months and 28 days after inoculation with the disease.

On Post Mortem two filaria equina were found in the peritoneal cavity. In the cæcum a few round worms, some 2 inches long, were found (not identified yet). In the stomach larvæ of the gad-fly (bots); and in the rectum oxyuvis rerumicularis were present. The liver was studded with numerous small calcareous nodules throughout these nodules were too hard to permit of making sections of the liver for microscopic examination.

Remarks. Only once after treatment did this horse's temperature rise a little above normal, when trypanosomes appeared in the blood, and then after further treatment they disappeared, and the temperature ran normal up to the time of death.

Horse D (see Charts 8A to 83). This horse was inoculated with trypanosome on 14th February, 1908, from Calf C. On the 24th February he showed trypanosomes in his blood; and on the 25th treatment was begun by daily intravenous injections of the methylene blue solution 5 c.c. diluted with distilled water 5 c.c.-this was continued for 10 days up to 5th March, but this had no definite effect on the temperature, nor did the trypanosomes disappear from the blood. On the eleventh

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day a larger dose of methyl blue, 10 c.c., was injected, but without effect, and therefore, on 7th March daily intravenous injections of 10 c.c. of perchloride of mercury solution (1 in 1,000) were begun and continued for 10 days. On the 8th March, the day after mercurial injections were begun, 4 c.c. of blood was inoculated into Dog L. On the 9th March, the second day after treatment was begun, the temperature fell, and on the 10th of March the blood on examination showed no trypanosomes. The temperature ran very nearly normal for 14 days after cessation of treatment, the blood showed no trypanosomes during this time, and on the 24th March 6 c.c. of blood were inoculated into Dog O. 31st March, again, a slight rise in temperature, with trypanosomes in the blood, On the occurred, and the mercurial injections were again made for two days, as will be seen by following the chart. The temperature now again ran a fairly normal course for 12 days, and the blood showed no trypanosomes when, on the 13th April, again a slight rise in temperature, with trypanosomes in the blood, occurred, and now the animal was again treated by injections of mercury for two days, after which the temperature ran normal for a month and a half, showing no trypanosomes in the blood. During this period, on the evening of the 15th May, 15 ozs. of blood were drawn off, allowed to stand in water at temperature of 80° F. during the night, and next morning the serum was injected subcutaneously into the following animals :-

Horse I Calf M Dog

Monkey B

dose 5 c.c.

11

30 c.c.

"

10 c.c. 10 c.c.

These animals will be referred to later

under Section III.

On June 1st Horse D again showed a slight relapse of temperature with trypanosomes in the blood, but no further treatment was given, and subsequently the temperature ran very nearly normal course, and no trypanosomes were seen in the blood, but the animal fell off in condition, and the chart will show that the morning temperature began to fall very low. With the onset of the wet season and the abundance of green grass the horse improved very much in general condi- tion. On the 23rd August, 1908, the temperature observations ceased, as the animal was ridden to Gujeba and returned on 3rd September, after 12 days; as a result of this constant work for 12 days the horse fell off very much in condition, notwithstanding good feeding. After his return from Gujeba the temperature observations were again continued, and the blood showed an absence of trypano- somes. He again improved in condition, and was, early in November, ridden down to Yola and back. (A march of 32 days.) He returned to 'Maifoni again reduced in condition, but improved with rest. My last information about the horse was dated early in January, 1909, when Dr. Alexander, with whom the horse had been left to be kept under general observation, wrote and said that the animal was doing well.

Remarks.-The further history of this animal will be interesting, as he is the only horse which has shown any signs of apparent recovery after treatment, so far as observations made during this time will permit of any such conclusions. If he survives and shows complete recovery he will be of value for the purpose of conducting further experiments on my return to the country after my leave.

Dog L (see Charts 8a to 8d). This dog was first inoculated with 4 c.c. of blood from Horse D on 8th March to keep up the strain of trypanosomes, but fearing it had failed because Horse D. had already been subjected to treatment by mercury (see Chart Sa), the dog was again inoculated on the 10th March with 6 c.c. of blood from Calf G, which was also under treatment by mercury (as will be seen later). Looking at the charts it will be seen that there was no definite onset of the disease during the incubative period of the first 12 days (see chart 5 A of Dog G). The temperature showed a slight rise occasionally, but the blood- although examined a great many times-never showed the presence of trypano- somes. From the 13th May to 30th June the temperature ran a normal course and trypanosomes were absent from the blood. After the 30th June the temperature observations were no longer continued, the dog gradually lost condition, and died on the 10th August-after a period of 5 months after inoculation.

Remarks. In this case we see that the dog did not develop any definite invasion of trypanosomes in the blood, although it was inoculated with blood containing trypanosomes from Calf G. It is possible that the effect of the mercury treatment of both Horse D and Calf G had attenuated the virus, and hence it failed to

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