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develop; but some toxic element must have existed in the blood of these animals which ultimately killed the dog after five months. Later it will be seen that many other dogs died ultimately, a considerable time after inoculation, although they showed no definite onset of disease by rise of temperature, and never showed any trypanosomes in the blood.

Dog O (see Charts 9a to 9d) was inoculated with 6 c.c. blood from Horse D. on 24th March, after the horse had been treated with the mercurial injections to ascertain if his blood was free from trypanosomes. Looking at the chart it will be seen that this dog showed no definite inset of the disease during the first 12 days, the temperature showed occasional rises, but the blood never showed any trypanosomes. On the 30th June temperature observations ceased; at this time the dog had become reduced in condition, and later on died on the 28th July. Duration of life after inoculation, 4 months and 4 days.

Remarks. It is evident that the blood of Horse D was rendered free from try- panosomes by the mercurial treatment, for this dog showed no signs of development of the disease and no trypanosomes in its blood, but it had irregular rises of tem- perature and ultimately died. It may be that the blood of the Horse D either contained some toxic material elaborated by the trypanosomes or some other phase of trypanosome which proved infective and fatal.

Horse E (see Charts 10a. to 10e),-This horse was inoculated and infected with trypanosomiasis and treated by injections of the mixture of methylene blue and perchloride of mercury at the same time as Horses C and D, for the purpose of comparing the therapeutical effect with the results obtained in the case of Horses C and D. On comparing the chart with that of Horse C, it will be found to bear a close resemblance to it, the effect of treatment being marked by a fall in the tem- perature and absence of trypanosomes from the blood, and to prove the latter Dog K was on the 4th March, or the eighth day of treatment, inoculated with 8 c.c of blood, but this dog never developed a definite onset of the disease nor did trypanosomes ever appear in its blood.

A month after treatment the chart will show two relapses in rise of temperature and re-appearance of trypanosomes in the blood, and at such times treatment by mercurial injections was repeated for a day or two-the tempera- ture falling after treatment and the trypanosomes disappearing from the blood. After the second relapse on the 9th April the temperature ran almost a normal course, later the morning temperature began to fall very low, the animal lost con- dition, grew very weak, and finally died on the 20th June, after a period of 4 months and 6 days, after being infected by inoculation.

Remarks. The results of treatment in this case resemble very closely those attained in the case of Horse C, in that the temperature fell, trypanosomes dis- appeared from the blood, relapses occurred; subsequently the temperature ran normal and no trypanosomes in the blood, but the animal wasted and died.

On Post Mortem. In the cœcum and great colon the following worms were found:-Amphistoma collensi, small worms resembling ankylostomes, in great numbers, and another round worm from one to two inches long-these two latter are still to be identified by Dr. Leiper, and a complete list of the entozoa found in the animals will be submitted later on.

Dog K (see Charts 11a to 11e).-On the 4th of March this dog was inoculated with 8 c.c. of blood from Horse E; on the eighth day of treatment by injections of methylene blue and perchloride of mercury.

From the chart it will be seen that there

was no definite onset of the disease after the period of incubation, and no trypano- somes were ever found in the blood when the temperature, which ran an irregular course, was high. On the 30th June the temperature observations were discon- tinued, and the dog was much reduced now in condition. He finally died on the 20th September, the duration of life after inoculation being 6 months and 16 days.

Remarks. The same remarks as those made on Dog O (see above) apply to this case.

The next two cases are Horses F and G, which were infected with the disease and then treated by injection of perchloride of mercury, only with a view to con- firming the results obtained in the case of Horse C.

Horse F (see charts 12a to 12i).--Was inoculated with 4 c.c. of blood, but as there were no signs of the horse having been infected by the eighth day, it was thought that the inoculation had failed, so on the 22nd March 6 c.c. of blood from Calf H, which showed numerous trypanosomes, was inoculated into the horse, with the result that the temperature steadily rose the next day, but showed no trypano somes, and then slowly fell. On the seventh day after the second inoculation the

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horse was found to be infected, the temperature rose, and trypanosomes appeared in the blood. Nine days later Calf I was inoculated with 6 c.o. of blood, and then treatment was begun by intravenous injections of perchloride of mercury solution (1 in 1,000) in doses of 10 c.c. daily, lasting for ten days, with the result that the temperature became normal and trypanosomes disappeared from the blood, and at this time Dog Q was inoculated daily with blood for three days. The total blood inoculated into Dog Q was 28 c.c. (see under Section III.). had a relapse of temperature and of trypanosomes in the blood, so he was again On 21st April Horse F treated for three days, after which the temperature again fell and the trypanosomes disappeared from the blood. From 5th May Calf view to noting if the blood had any curative value, but it proved a failure, as the was inoculated daily with a calf died subsequently. The total blood injected into Calf 13th May inoculations of blood as a prophylactic into Calf K were begun, but the was 765 c.c.. From next day, owing to a sudden very high rise of temperature in the calf, it was not done, but continued on the following day for a further period of four days, the total quantity of blood injected into Calf K being 88 c.c. (see Section III.). On the 23rd May Dog W was injected with 10 c.c. of blood as a prophylactic. On the 26th May Horse Fhad a relapse of temperature and of trypanosomes in the blood, but no further treatment was adopted. The subsequent history of Horse F is that the tem- perature ran normal for some time and no trypanosomes could again be found in his blood. In August the animal fell off in condition, and the morning temperature fell very low; in September there was a slight rise of temperature, but no trypano- somes in the blood. During October the afternoon temperature continued to rise daily to near 102° F., but still no trypanosomes were observed in the blood. On the 31st October the temperature observations ceased, the horse was very poor in con- dition, although he had improved somewhat with the onset of the wet season and the abundance of green grass; the outlook, however, was not favourable. The animal was left at Majfoni with Dr. Alexander to be kept under general observation when I left Maifoni early in November, and his ultimate fate is not known to me.

Remarks. The effect of treatment by mercury in this case resembles that achieved in other horses similarly treated, viz., the fall in temperature with dis- appearance of trypanosomes from the blood, followed later by occasional relapses in temperature with or without trypanosomes appearing in the blood, and then finally a general falling off in condition.

Horse G (see Charts 13a to 13c). This animal was not in a very strong con- dition when experimented on.

On 14th March he was inoculated with 4 c.c. of blood

from Calf H, but fearing this had failed he was re-inoculated (as in the case of Horse F) with 2 c.c. of blood from Calf H on the 22nd March. On the 25th March the animal was found to be infected, and on the 27th treatment by injections of perchloride of mercury solution (1 in 1,000) 10 c.c. daily were begun and continued up to the tenth day. On the 26th March, before treatment was begun, 6 c.c. of blood were inoculated into Dog N. On the 26th March, when the blood was examined and the horse found infected with trypanosomes, it was found that the blood con- tained a non-sheathed embryo of filaria equina. The effect of treatment was to lower the temperature and remove the trypanosomes from the blood temporarily, but not the filarial embryos. On the 19th the temperature again rose and trypanosomes appeared, the animal was again treated by mercury, with the same result. time 7 c.c. of blood was inoculated into Calf J,

At this

27th April, and he was again treated by mercury, but the effect was not so good as Again a relapse occurred on the before, and trypanosomes continued in the blood, the animal grew weaker, and finally died on the 11th May.

On Post Mortem the only notable feature was the large amount of fluid in the pericardium.

Remarks. This animal's condition not being very good at the onset, treatment was not so effective, relapses more common, and a fatal termination much more rapid. Calf I (see Charts 14a to 14c).-Was inoculated with 6 c.c. of blood from Horse F on the 6th April, and developed the disease by the 12th without showing any very marked rise of temperature above the normal. practically normal, but the blood continued to show the presence of trypanosomes The temperature continued to run while the treatment by daily intravenous injections of perchloride of mercury solution (1 in 1,000) in doses of 10 c.c. was being given. found in the blood the day after the last injection, the treatment was continued for As the trypanosomes were still two more days, but was of no avail, for it had no effect on the trypanosomes in the blood, but the temperature continued to run a low course throughout. On the 4th

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