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from the charts it will be seen that repeated examinations of the blood made right up to the time of death on the 26th June gave negative results as to presence of trypanosomes. On the 3rd May the blood was found to contain a filarial embryo identical with one of the forms of filarial embryos found in the blood of Horse G, next to which this horse was always tethered. The filarial embryo resembled the F. Perstans. It was non-sheathed and sharp-tailed. This embryo was identified in blood films from Horse G as the young of Filaria Equina by Dr. Leiper, Helmin- thologist to the London School of Tropical Medicine. On 23rd June horse too weak to stand.

Death and Post Mortem Examination.-On the night of the 25th June the horse, which had gradually lost all flesh and was nearly a skeleton, died, and on post mortem eight slender round worms about 4 inches long were found in the peritoneal cavity; in the large intestines (colon and cœcum) many small worms resembling ankylostomes and a few Amphystoma Collensi. The eight long slender worms found in the peritoneal cavity were identified by Dr. Leiper as the Fitaria Equina.

Duration of Life of Horse B after inoculation followed by treatment was 7 months and 28 days.

Remarks. The effects of treatment in this case were (1) to remove the trypano- somes from the blood, and that recurrence of trypanosomes in the blood was only known to occur once after treatment and then to have disappeared with- out any further treatment being administered to the animal. The inocula-

tion of 10 c.c. of blood after treatment, when the trypanosomes were absent, into Dog E did not reproduce the disease in the dog, as will be seen in the description of the case following. (2) The temperature kept fairly normal after treatment; but (3) the horse gradually lost condition and died after being ill for 7 months and 28 days. The presence of embryos of F. equinu in the blood was only learnt on the 3rd May; it was never found in the repeated examinations of the blood made before this date, and was probably acquired while under experiment. How far the filarial infection aided à fatal termination is difficult to say.

Dog E (see Charts 2, A, B, and C).-This animal was on the 19th November, 1907, injected with 10 c.c. of blood from Horse B after his treatment, when the horse's blood was microscopically found to be free from trypanosomes. On the 30th December, 1907, when the former report was submitted, the animal was in good health and had not shown any signs of disease, nor any invasion of the blood with trypanosomes after the usual period of incubation, although there were some rises of temperature after inoculation of the blood. After 1st January, 1908, up to 14th March, the temperature ran a normal course and repeated examinations of the blood showed no trypanosomes. In January this bitch was accidentally lined and became pregnant. On the 14th March the temperature was no longer taken owing to her having given birth to seven pups. On the 30th April she was in good health and condition, but was accidentally shot by me at night while engaged in a fight with a stray dog.

Remarks. After inoculation with as large a quantity as 10 c.c. of blood from Horse B, this dog did not show trypanosomes in its blood at any time and was never ill; and at the end of 5 months and 10 days, when she was shot, was in good health and condition, so that the blood of Horse B was apparently free from try- panosomes as such at the time of inoculation, and that the effect of the treatment of Horse B by intravenous injections of methylene blue and perchloride of mercury had been to remove the trypanosomes, as such, from the peripheral blood. Three of the seven pups were kept and allowed to grow up. They were quite strong and healthy and did not show any signs of trypanosomiasis.

Calf (see Charts 3a to 3d).-No report was made on this case before this. This calf was on the 30th November, 1908, inoculated with 8 c.c. of blood from Calf B which had been treated by intravenous injections, and when the blood was apparently free from trypanosomes; but by the 14th December, 1908, this calf developed trypanosomes in its blood, and next day, on the 15th, treatment by intra- venous injections of methylene blue and perchloride of mercury were begun and continued for 10 days, up to the 24th December. On the 25th December the blood was examined hut found to contain trypanosomes, so the treatment by injections was continued for another five days, and on the 30th December the blood on exami- nation showed no trypanosomes. The temperature, however, ran high, and on the 18th January, 1908, the blood showed trypanosomes. On the 19th January, 1908, 5 cc. of blood was inoculated subcutaneously into Calf C and now the calf was subjected to treatment by intravenous injections of methylene blue, 10 c.c. daily,

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up to the 29th January, 1908. On the 30th January the blood on examination showed no trypanosomes. On the 31st January also no trypanosomes were seen in the blood, and 10 c.c. of blood were inoculated into Dog II. The calf was now treated by intravenous injections of perchloride of mercury solution (1 in 2,000), 10 c.c. daily, for 10 days. Subsequently, although no trypanosomes were found in the blood, the temperature continued to run high; finally, on 1st March, the animal was very weak and unable to sit up. Death occurred on the morning of the 5th March.

Calf E (see Chart 4).—This 'animal was inoculated with 8 c.c. blood from Black Government Ox, No. 2, on 30th November, 1907, and treated by injections of methyl- blue and perchloride of mercury, details of which are in my previous report; but trypanosomes continued in the blood after treatment, the calf grew worse, and the case ended fatally on the 19th January, 1903.

Dog G (see Charts 5a and 5b).-This dog was infected by inoculation of 1 c.c. of blood from Black Government Ox, No. 2, on 5th December, 1907, and in the course of 11 days developed the disease.

Remarks. No treatment was administered to this dog, but the chart has been added as there are certain points of interest in the case.

(i) The temperature chart presents a fever of a definite relapsing type. (ii) The trypanosomes were present in the blood both when the temperature

was high and low.

(iii) The mode of death was in convulsions and coma, the two conditions

alternating and lasting for four hours before death.

Dog I (see Charts 6 a to 6 g) was infected with trypanosomes by inoculation of 1 c.c. of blood from Calf D on 19th January, 1908, and first showed trypano- somes in his blood on 31st January, but treatment by intravenous injections was not begun until the 17th February, much later than most of the other cases experi- mented on. Treatment was continued for 10 days up to 26th February, after which the temperature ran a low and irregular course and the blood occasionally showed the reappearance of trypanosomes in it, but a rapid disappearance also. The dog died ultimately on the 26th July; on post mortem the spleen was found very much enlarged and the contents semi-fluid, the liver a pale yellow and soft.

Duration of Life.-Life in this case was very much prolonged after inoculation, the animal living for a period of 6 months and 7 days.

Summary of the results of treatment by methylene blue and perchloride of mercury, including the cases treated in 1907 and already reported on:- Two Dogs, A and B, treated by subcutaneous injections, but both died. Dog I, treated by intravenous injections, result eventually fatal but life very considerably prolonged. Trypanosomes occasionally reappearing in the blood after Two Horses, A and B, treated by intravenous injections, but both died. In the case of Horse B life was very considerably prolonged, and the blood apparently rendered free from trypanosomes (see pages 53 and 54).

Four Calves, A, B, D and E-Treatment a failure in all four, as all ended fatally.

treatment.

Two Government Oxen Nos. 1 and 2.-Treatment a failure, as both died. Under Section II. it will be seen that another case, Horse E, treated by the above mixture recommended by Mr. Brand terminated fatally (see page 58).

Of the above 12 cases treated, three cases (viz., Dog A and Oxen 1 and 2) were cases of natural infection, the rest were cases of trypanosomiasis by inoculation.

Section II.

Consisting of cases experimented on with a view to making a therapeutical analysis of the ingredients composing Mr. Brand's mixture, and ascertaining the value of the methylene blue and the perchloride of mercury individually on the disease.

To carry out the above three horses, viz., Horses C, D), and E, were taken, and a series of experimental treatment conducted on them at the same time for the sake of comparison; in all three the treatment was begun as soon as infection was found to have taken place after inoculation.

The first case, Horse C, was treated by intravenous injections of perchloride of mercury only.