65
TABLE IV.
Agglutina
-It is agglutinated by cholera serum. by Strain I. and Strain II sera.
Strain III.
It is well agglutinated
This strain was isolated from the water of a well in Matale, near which a small epidemic of cholera-like cases occurred. Unfortunately no stools of the cases were sent to me, but a sample of water collected with all precautions and packed in ice was sent to me. The well, being considered to be the cause of the epidemie, was closed, and the epidemic ceased.
Microscopical.—The vibrio is morphologically similar to Strains I. and II. Cultural characters.-These are collected in the following table :-
V. Hagallensis (Strain 114.)
Litmus Milk,
TABLE III.
Leotass
Bscobarom.
Daichte.
Mannita.
Giacom
Maltors.
Dextrine.
Ar Pe Ave A
A
Indel.
AATE
Voges Froak.
Babinose.
012
V. Kegallensis, Strain L
V. Kegallensis, Strain II.
V. Kegallensis, Strain III. + As Po Ave
V. Insolitus
True cholers, Strain 1
(Baigon)
+ +
Kanuite.
OBDOBIA
Maltom.
Dextrine.
Rafinose.
Arabinose.
O
❤
Adouite
Lauliu.
Galactose.
Borbita
"BBOTTABAT
•
+ A Po
A
O
A
Ava❘ 0
→
A
Alk
0
+A Po
A A
0
A
A A
A
Alk
0
A 0
A Ave
A
0
olá o
01
All Alk
A
AvE O
A
Alk
As Pc Avs
-
O
-
A A A
0 A 0
O
A
As
A 0 A A
A Avs 0 0 0
A 0
012
00130
A
Alk
A
Alk
AVA
0
+
A
0
A
A
A
A
0
Alk
Alk
True obolers, Strain 8
+
A
0
Alk❘ A A A
ov
A
0
A
Avs Alk Ave | Alk| Alk| A Alk A
(Ceylon)
A
True cholera, Strain 4 + AC A
A
Alk A
A A
A
All O Alk Alk A
All A
(Ceylon)
True abolera, Strain 5
+
AO As
A
All A
A
A
0
Alk Alk Alk| Alk| A
All A
(Ceylon)
Peptone Water.
True cholera, Strain 6 + A
(Ceylon)
Απ A
ΔΙΚΤΑ
A A
Ave Alk Ave Alk Alk A
Alk A
0
True cholers, Strain 7
(Ceylon)
+
A
A
0 Alk A A A
Alk
Alk Alk A All A
Ávi
Ava
Ambínom.
#sinalom. Inalia.
Adonita.
orbita
Leerglass.
•
0 Ava 0
True cholers, Strain 2
(Ceylon)
93104
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Inosite.
Saliotne.
Amygdalin.
Jundulaite.
V. Kagallensis (Strain IIL)
0
O
O
❤
Erythefte.
Glycerine.
•
O
O
Neutral Bed.
German.
Celtias.
Ó
*NOTRANJË “VERZE
+
+GTP T
Abbreviations used in the table:-A, soid; C, clot (milk), clear (broth and peptone water); T, tur- bidity; P, pellicle (broth), peptonized (milk); a slight; vs, very slight; Po, upper portion peptonised and cloé si the bottom; negative, then alkaline; O, negative result: vix., neither acid nor alot in milk, neither said nor gas in sugar media, non-production of indol, nan-liqualration of gelatine or serum, as the case may be; +, positive result: liquefaction of medium.
Agglutination.—It is not agglutinated by cholera serum, while it is agglutinated by Strain I. and Strain II sera.
Remarks on the Cultural Characters of above Strains and the true Vibrio Cholera. The cultural characters of the three strains are practically identical with each They are culturally, other, though minor inconstant differences may be noted. extremely similar to, or identical with, various strains of true cholera. In Table IV. are collected the cultural characters of the three strains of Vibrio kegallensis, the cultural characters of a cholera-like vibrio found in canal water in Colombo (Vibrio insolitus, Cast, 1913) biologically different from V. kegallensis and V. cholera, and the cultural characters of six strains of biologically true cholera derived from various sources. It will be seen that the three strains of V. kegallensis did not produce indol, but in my experience one comes occasionally across a strain of true cholera which only produces a very slight amount of indol or none at all. It will be seen also that Vibrio kegallensis as a rule does not produce a very distinct pellicle in peptone water, but in my experience this is also the case, though rarely, with certain strains of true cholera. As regards the production of hæmolysis, this is inconstant with all the strains of V. kegallensis.
V. Kegallensis, Strain I.
V. Kegallensis, Strain II.
V. Kegallensis, Strain III.
V. Insolitus
00000
Inosite.
2100101 Balicine.
0
A
0001
O O O O
O
Amygdalin.
Isoduloite.
Alk Alk
+
Alk Alk
0
True cholars, Strain 1
(Saigon)
True obolera, Strain 2
(Ceylon)
O
O
O
Erytbrite.
Glycerine.
•
Indol
O
O
Voges Prosk.
Beda. Nitrates.
Neutral Red.
Gram.
Gelatine,
Barum.
O
0
O
O
O
O
0
For
0 0
0 0 0
0
0
O
Alk Alk
AVB
0
O Ava +
0
0 0
0
0
O
True cholers, Strain 8 Alk| Alk| Alk| Alk Alk O
(Ceylon)
+
+
0
O
❤
0
O
Broth.
Peptone Water.
+ +
T
T
+
+
GT P
T
+ + GT P
T
+
GT P
0
0
+
O
O
+
+
O
party
+
+
T
GT P
T
+ GTP GT P
A
A
Alk
0
+
+
ÁVE
0
0
+ +
GTP
0
+
+ +
GT P
P
0
+ 0
+
+
GT P
P
0
0
0
+ +
GT P
P
0
+
0
+
+ GT P
P
True cholers, Strain 4 Alk| Alk| Alk| Alk Alk O
(Ceylon)
True oholera, Strain 6 Alk Alk Alk| Alk| Alk] Alk
(Ceylon)
True cholers, Strain 6 Alk| Alk| Alk| Alk Alk O
(Ceylon)
+
+
True cholers, Strain 7 | Alk Alk| Alk Alk Alk Alk+
(Ceylon)
+
O
A
Abbreviations used in the table:-A, acid; C, clot (milk), clear (broth and peptone water); T, tur- bidity; P, pellicle (broth), peptonized (milk); s, slight; vs, very slight; Pc, upper portion acid then alka peptonized and clot at the bottom; negative then alkaline; line; Alk, alkaline; GT, general turbidity; O, negative result; viz., neither acid nor clot in milk, neither acid nor gas in sugar media, non-production in indol, non-liquefaction of gelatine or serum, as the case may be; +, positive result: liquefaction of medium.
G