PUBLIC RECORD OFFICE
T[ :། ། ། ། T
Reference :-
CO. 885
23 PUBLIC RECORD OFFICE, LONDON
ALLY WITHOUT PERMISSION OF THE BE REPRODUCED PHOTOGRAPHIC- COPYRIGHT PHOTOGRAPH-NOT TO
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SUPERINTENDing Medical Officer,
Christiana, 7th December, 1914. I HAVE the honour to acknowledge the receipt of Circular 90, No. 3419, dated 3rd December, 1914, and in answer to state that I have treated no cases of yaws with salvarsan.
5626
(No. 14.) SIR,
No. 99. CEYLON.
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TABLE I.
W. G. FARQUHARSON,
District Medical Officer.
Litmus Milk.
Lactose.
A
V. Kegallensis (Strain L.) .
A. Po
0
Alk
THE GOVERNOR to THE SECRETARY OF STATE. (Received 4th February, 1915.)
The Queen's House, Colombo, Ceylon, 11th January, 1915. WITH reference to my despatch No. 459, of the 3rd August, 1914,* I have the honour to forward herewith, for transmission to the Advisory Committee of the Tropical Research Fund, a report by Dr. Aldo Castellani on research work carried out at the Clinic for Tropical Diseases and Bacteriological Institute during the half year ended 31st December, 1914.
I have, &c.,
ROBERT CHALMERS,
Governor, &c.
Enclosure in No. 99.
Report on the RESEARCH WORK CARRIED OUT at the CliniC FOR TROPICAL Diseases AND BACTERIOLOGICAL InstitUTE DURING THE HALF YEAR Ending 31st DECEMBER, 1914.
THE routine work has been heavy at both institutions: the number of speci- mens examined at Bacteriological Institute exceeding 1,500 for the six months. During my spare time I have carried out some investigation work on the following subjects:
1. Paracholera.
2. The internal treatment of yaws.
3. A case of triple infection: typhoid-paratyphoid A-paratyphoid B.
4. A case of vaginitis due to Monilia pinoyi, Cast.
5. Combined vaccinations.
V. Kegallensis (Strain L)....
Inosite.
0
Salicine.
O
O
O
O
04
+
Amygdalin.
Isodaicíte.
Krytbrite.
Glycerine.
Indol.
Voges Prosk
Reda, Nitraten.
Neutral Bed
Gram.
Gelatine.
Gaocharose.
Dulcite.
Manoite.
Glucoen.
Maltose
AVI O
A
0
Dextrine.
Ballbore.
Arabinose.
Adonite.
O
Inulin.
+
Berum.
►
Galactone
T
Broth.
•
Borbite
T
Peptone Water.
A
Leerulone.
OA
•
Abbreviations used in the table:-A, acid; T, turbidity; va, very alight;
A
AE,
negative, then acid; acid, then alkaline; Pc, upper portion peptonised and clot at the bottom; O, negative result: vis., neither said nor clot in milk, neither acid nor gas in sugar medis, non-production of indol, non-liquefaction of gelatine or serum, as the case may be; +, positive result: Liquefaction of medium.
It is
Agglutination. This vibrio is not agglutinated by true cholera serum. well agglutinated by Strain I., Strain II., and Strain III. sera.
Pathogenicity. I have not succeeded in reproducing the disease in the lower animals.
Strain II.
This strain was isolated from the intestinal contents of a native who died after a short illness characterized by serous diarrhoea, vomiting, severe muscular cramps, etc.,
like true cholera.
Microscopical and cultural characters. Microscopical: the vibrio is morpho- logically similar to Strain I. Cultural characters: these are collected in the following table.
TABLE II.
PARACHOLERA.
In a previous report I gave a brief description of a vibrio I found in a case of paracholera. I have found the same vibrio in a further case, and also in the Of this water of a well near which several cases of cholera-like disease occurred. vibrio, which I have called V. kegallensis, I have, therefore, three strains, which I I have been able will designate as Strain No. I., Strain No. II., Strain No. III.
out a fairly complete investigation of the three strains, and comparative to carry researches with true cholera.
Strain I.
The strain was isolated from the stools of a patient in Kegalle showing all the symptoms of cholera; profuse serous diarrhoea; severe muscular cramps, etc. The microscopical and cultural characters of the germ are as follows:-
Microscopical.-Films from the stools stained with diluted fuchsin contained numerous vibrios, the same shape as the true cholera one, but perhaps slightly larger and thicker.
Motility. The vibrio is very motile, like the cholera vibrio.
Staining reactions.-Easily stained by the usual aniline dyes. Gram negative. Cultural characters.-On agar and gelatine the growth has a certain resem-
Serum is also liquefied. blance to true cholera. Gelatine is liquefied.
Milk is
rendered acid and clotted. Table I. gives the principal cultural characteristics and sugar reactions of the micro-organism.
* No. 5 in Appendix VIII. to [Cd. 7796].
Litmus Milk.
Lactose.
Baccharose.
Dulcite.
Hannite.
Glucose.
Maltone.
V. Kegallensis (Strain II.)... A. Po A
V. Kegallensis (Strain II.),....
A
Inosite.
Salicine,
Amygdalin.
Inodulcite.
Alk Alk
0
O
Erythrite.
Glycerine.
Indol.
о
O
O
O
0
Dextrine.
A A
Rafinone.
Laevulose.
Alk
Alk Alk
ola
013
Arabinose.
Adonite.
Inulin.
D-
Galactose.
Alk
A
012
Borbite
Voges Prosk
Redn. Nitrate.
Neutral Red.
Gram.
Gelatine.
O
0
+
O
Berum.
Brotb.
Peptone Water.
+GTP T
Abbreviations used in the table:—A, acid; T, turbidity; negative, then alkaline; C, clot (milk), clear (broth and peptone water); P, pellicle (broth), peptonized (milk); s, slight; Po, upper portion peptonized and clot at the bottom; O, negative result, viz., neither acid nor clot in milk, neither acid nor gas in sugar media, non-production of indol, non-liquefaction of gelatine or serum, as the case may be; +, positive result: liquefaction of medium.
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