:

1

September.

October.

සු

C.

Number

of Goat.

M.

801

101

111

112

+11

1

113

117

C.

108

Agar plates were smeared with material taken from spleen, liver, kidney, inguinal glands, mesenteric glands, heart, and bladder.

Numerous growths were obtained from No. 16 from spleen, liver, inguinal glands, and mesenteric glands.

From No. 108 a few colonies were found on the plate smeared with a portion of kidney, all the other plates showed no Micrococcus melitensis growth.

None of the other kids gave colonies of Micrococcus melitensis.

13. The growths from No. 108 were quite unexpected, as the kid had never reacted to Micrococcus melitensis during life, and as no growth was obtained from the spleen and glands, which are much more likely to contain the microbe.

Still, the infection of this kid could in no way be compared with that of No. 16, which gave pure cultures of Micrococcus melitensis in a large number of plates, and which had shown a good serum reaction during life.

14. Of course, the finding of the few colonies in No. 108 does not allow one to affirm that the kids born of infected mothers had acquired immunity, but taking all the circumstances together, one can easily see that the four kids bought out of the Lazaretto suffered much more severely than the others. Nos. 7, 16, and 17 gave clear reactions all the time, whilst of the Lazaretto kids the serum reaction was never evident.

15. In conclusion I think that this experiment gives us a primâ facie evidence that kids born of infected dams have a certain degree of immunity to the Mediter- ranean Fever infection.

16. If the experiment be repeated on a larger scale, and my conclusions confirmed, a clue to the grave problem of freeing the island from infected goats may have been found, as it will be comparatively an easy question to replace infected goats by immune kids.

January 31, 1908.

TEM. ZAMMIT.

July.

March.

April.

May.

June.

C.

M. B. C.

M. B.

C.

M. | B.

C.

1.

TABLE "A." SHOWING STATE OF GOATS FROM SEPTEMBER, 1906, TO AUGUST, 1907.⚫

December.

November.

C.

M.

; 1

p

C.

January.

C.

February.

3

C.

-

+|++++|++ +

+

+1+++1+

655555555 F

+8++1++++|+

DERBEBEBEBÊ

+++++++++

+ 11+ +

+ ++1+1 +

ཙྩâgརེཏྟི།།gཏི།

++++H++++|+

1སྦྱིཎྚཀཽ།། iz

+++++++++++

1

++++++ +

སྐྱདྡྷིཥྚ་༄འིཙྪ་ཧྰུྃ།

+++++++++++

|| ++++++ +

+1++++++

+

+i++1+

H

August.

C.

11. B.

2.

1

+++++1+

+1+14+

+1

1

+|++++!+

1

+1+1++

+1

+1+1+1+

+1

*2

193

+

+1++++1+

+

+1+++

+

++++++1+

11

+

+

++++

+1

+ 1 ++ ++ 1 +

REMARKS.

No. 113 dropped 4 kids on 31st March, killed on 29th August. No. 115 died on 26th January, M.M. recovered at post-mortem. No. 111 dropped 3 kids on 8th March, killed on 29th August. No. 117 killed on 8th August.

No. 101 dropped 1 kid on 15th February, killed on 29th August.

No. 104 killed on 8th August, M.M. recovered at post-mortem,

No. 107 killed on 9th Angust.

No. 111 dropped 1 kid on 6th February, killed on 29th August, M.M. recovered at post-mortem, No. 110 dropped 2 kids on 10th February, killed on 16th August, No. 112 dropped 2 kids on 11th February, killed on 29th August,

No. 108 dropped 2 kids on 27th March, killed on 29th August.

100

1411

+

+ stands

for positive, — for negative, for doubtful, reaction. N.B.-M. stands for milk, B for blood, and C. for Colonies per

per i c.c. in milk.

PUBLIC RECORD OFFICE

Reference :-

TTTT C.O.885

19 PUBLIC RECORD OFFICE, LONDON

ALLY WITHOUT PERMISSION OF THE BE REPRODUCED PHOTOGRAPHIC- COPYRIGHT PHOTOGRAPH-NOT TO

ตน น

TABLE B." SHOWING BLOOD REACTION OF KIDS FED ON INFECTED MILK.

June

Link

July

26th.

2nd.

12th.

17th. 21th.

September.

17th.

23rd.

October.

3rd.

10th.

*3*1

- Killed

Killed

-

с

110

Killed

Killed

Killed

Killed

Killedi [Killed

Kille J

Killed

+ Killed

+11++1

15th February, 1907 ... 10th February, 1907 ... | 27th March, 1907

***

1907 ...

6th February, 1907 ... |

11th

31st March, 1907 31st March, 1907 31st March, 1907

8th March, 1907

Bought June 19th Bought June 19th Bought June 19th Bought June 19th Bought June 19th

...

...

9,9+

+1 +

7.

+ | | | | | |+|{

Died

1 1++1

+1++

N.B.-Infected milk was given to all kids only twice between 11th June and 2nd July.

Kid No. 108-few colonies of M.M. in plate from kidney.

- --

+++

-2-2-2-2

+1 +1 +1 +1

#1 | | 1

5-3-3-

+1 +1 +1 1

Fractions denote dilation of blood serum, + stands for positive, and — for negative, reaction, ± for doubtful reaction. Kid No. 16-badly infected-M.M. in spleen, liver, inguinal glands, and mesenteric glands.

February.

Number of Kid.

Date of Birth at

March

Lazaretto.

4th.

20th.

101

...

...

...

...

...

...

***

...

...

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