PUBLIC RECORD OFFICE
Reference :-
ITT C.O.885
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19 PUBLIC RECORD OFFICE. LONDON
106
At the post-mortem examination the animal looked normal and very fat, the Veterinary Surgeon declaring it a first-rate sheep, although he knew that it had yielded Micrococcus melitensis freely in the milk for months. A few sublumbar 'glands were found congested, and even hæmorrhagic in points, though not much enlarged. All the other glands looked normal, but enlarged.
7. The same can be said of goat No. 117. Animal kept lively and well all through, and gave a first-rate carcase, although the Micrococcus melitensis was recovered in great abundance from all glands (inguinal, mesenteric, and mammary).
Goat No. 115 died in January, 1907, and as putrefaction had set in when it was brought over to the laboratory no post-mortem was possible. It had been ill for some time and had dried up.
9. Goat No. 104 had dried up at an early date, and had remained barren. No Micrococcus melitensis had been got from its milk before it dried up, and its blood had ceased to react. At the post-mortem examination it looked quite normal, and no Micrococcus melitensis was obtained from any of the organs examined. For all intents and purposes the goat looked cured of its attack of Mediterrancan Fever.
10. The rest of the goats had a regular pregnancy, and dropped from one to four kids in due time. One of them (No. 114) died of septicemia after dropping
three kids.
11. The goats suckled their kids and kept quite well. Their blood and their milk reacted, more or less, all the time, except for No. 112, which persistently gave no reaction although Micrococcus melitensis was recovered from its milk twice in March (100 and 50 colonies per 1 c.c.), and once in May (39 colonies per 1 c.c.).
12. At the slaughtering of these goats, 16 months after their being discovered to be infected, and 6 months after delivery, the infection had greatly subsided.
13. Nos. 101 and 112 yielded no Micrococcus melitensis at the post-mortem examination, though No. 101 had Micrococcus melitensis in the milk in April (15 colonies), and its blood had shown throughout a positive reaction.
14. No. 110 gave also negative results at the post-mortem examination, but its blood showed a strong reaction, and colonies of Micrococcus melitensis were abundant in its milk not earlier than May and June (69 and 300 colonies per 1 c.c.).
15. No. 113 was the most curious of the set. Its milk and its blood had persistently reacted throughout, and for about a week in June (two months before the post-mortem) had yielded as much as 200 colonies per 1 c.c. of milk, yet no Micrococcus melitensis could be recovered from any of its organs.
16. The only goat that showed Micrococcus melitensis at the post-mortem was No. 111, though even this in a very unexpected manner. The animal had given positive reaction both in the milk and in the blood during life, and had started yielding Micrococcus melitensis in the milk since February, 1906. No Micrococcus melitensis was recovered in the milk from April to June 12th, but up to June 22nd At the post-mortem the colonies were very numerous, surely over 1,000 per 1 c.c. Micrococcus melitensis was obtained only from the inguinal glands.
17. From these observations I think it is fair to conclude that goats once infected with Mediterranean Fever, very rarely, if ever, shake it off, and also that infected goats are a constant danger, as they are apt to yield infected milk unexpectedly.
13. Another very interesting observation we made is that although as a rule an infected milk reacts when tested like blood serum for agglutination with Micro- coccus melitensis, cases were observed when a milk full of micrococci failed to give the agglutination reaction.
19. One feels also bound to remark that of the eleven goats examined, Nos. 107, 111, and 117 only gave colonies of Micrococcus melitensis, and of these Nos. 111 and 117 had been treated with injection of dead colonies of Micrococcus melitensis about a year previously. The goats thus treated were Nos. 104, 111, 114, and 117.
Enclosure 2 in No. 56.
REPORT on the Observations made on Kids Fed on Infected Milk.
1. Seven infected March, 1907:-
goats dropped kids at the Lazaretto in February and
Goat No. 101 dropped 1 kid.
103
2
2.
107
Goat No. 110 dropped 2 kids.
23
"
"
"1
111 112 113
1
"
"
2 4
114
"
#1
"
3 (1 dead in utero)
When more than one kid was born, one kid was killed immediately and examined. When only one was born it was allowed to live for further observations.
3. The kids were given the number of their mother so that the case could be followed more clearly.
4. The kids were allowed to suck their mothers, and only when the
the kids were suckled by another goat.
5. Table B" shows the state of the kids during the experiment.
goat died
6. The main point to be observed was as to whether a kid born of an infected For this mother would acquire immunity to Micrococcus melitensis infection.
five kids were bought of approximately the same age as those born at the purpose, Lazaretto (about 4 months) for the purpose of comparison.
7. The Lazaretto kids were left with their dams for nearly four months, when they were able to feed on clover, cotton-seed, and beans.
8. At this time all the kids were given milk from an infected goat in two- doses, one draught in the evening and one next morning. After this they were finally separated from the dams.
9. As at the time both goat No. 111 and 113 yielded infected milk, those two goats were destined to give the milk for the experiment As, however, goat No. 113 stopped to give infected milk quite suddenly, to be on the safe side all the kids fed on No. 113 milk were fed again twice on the milk of goat No. 111, which contained Micrococcus melitensis as before.
10. All the kids killed and examined soon after birth were found to be free from infection; their blood never reacted, and no Micrococcus melitensis was recovered from their organs and glands. Not one of the kids showed reaction in the blood immediately after birth; but some of them developed the reaction soon after.
11. The parallelism of the reaction of kid and mother was not maintained.
February 20th.
February 26th.
March 3rd.
April 16th.
GOATS.
101
+ 1 100
+ 180
110
+ 1 100
+ 1 50
+ 1 160
111
+ 1 100
+1.20 + 1
+ 1 100
+ 140
20 + 1 20
+ 140
+ 1 20
112 ...
+ 1,20
KIDS.
101
+ 150
+ 160
+ 1 100
+ 1 20
110
+1.40
111
112
+ 140
+1 100
1,20
+ | | |
+111
12. Two months after their birth all the kids showed no reaction in their blood up to their death.
In August three of the kids developed diarrhoea and were nearly dying when they were killed and carefully examined.
No. 101-Bowels congested, mesenteric glands enlarged.
No. 114-Intestines inflamed, pericardium full of serum, mesenteric glands enlarged.
No. 22-Intestines highly congested, stomach full of undigested food, gall bladder distended with glary bile, kidneys much congested.
No Micrococcus melitensis was obtained from any of the organs.
Between the 1st and the 10th of October, all the kids were slaughtered and carefully examined. They all appeared in perfect condition and moderately fat. The organs in all looked perfectly healthy, the spleen normal, except in Nos. 16 and 17 in which it was friable and juicy.
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