M 75
(8) Friedmann test for pregnancy.-Ten Friedmann tests were carried out during the year.
(9) Miscellaneous tests. One thousand five hundred and twenty-three examinations of various kinds not listed otherwise were performed. This includes certain work of a research nature:
229 B. typhosus agglutinations
1,000 Ide serological tests
82 Diaphragm digestions for trichinae
212 Other unspecified tests.
D. PREPARATION OF VACCINE LYMPH.
The preparation of lymph was much accelerated this year. In addition to the low level of stocks held, the onset of hostilities in China and the presence of large numbers of refugees led us to build up a good reserve so as to be ready to cope with any demand which the presence of smallpox during the winter might very well call forth.
The seed used was as before one generation removed from lapine, i.e., lapine, calf, seed. The yield on the whole was good, although we had several calves which were not up to a good physical standard and consequently gave poor individual yields. Forty-eight calves were used and 7,411 g. of pulp was obtained; a yield of 154.39 g. of pulp per calf.
The technique in use at present has been strikingly successful in improving the yield of pulp and thereby lowering the cost of production of the finished lymph. This is attained without any loss of potency or other change in the quality of the lymph. We feel that we can hardly expect any further improvement in this respect for some time...
The appended table shows the yield of pulp year by year for the past few years.
Table XLI.
Date No. of calves scraped for pulp Total pulp collected Average yield per calf 1931 83 2,163 gms. 26.06 gms. 1932 122 4,160 34.09 1933 148 5,787 39.10 1934 116 5,816 50.14 1935 73 nil nil 1936 4 622 155.50 1937 48 7,411 154.39Amount of lymph prepared
28,065 c.c.
Amount issued
22,651 c.c.
in stock at end of year
18,477 c.c.