M 132
35. The mosquitoes were brought to the Laboratory, blood taken from the midguts and sent to Dr. Toumanoff, Chef du Laboratoire d'Entomologie de l'Institut Pasteur de Saigon, who kindly had the samples examined for precipitin reactions. The results are given in Table IV.
36. The salivary glands of 712 A. maculatus whose midguts had been used for precipitin preparations, were examined for sporozoites, but none were found. No infections were found in the salivary glands or midguts of another batch of 597 A. maculatus.
37. Catching operations were continued during 255 nights in 1936, 5643 Anophelines were captured, 1377 dissected and 116 examined for precipitin reactions at the Laboratory of the Bureau. Of those caught 5589 were A. maculatus, 46 A. hyrcanus, 42 A. minimus, 1 A. fluviatilis, 10 A. jeyporiensis, 1 A. splendidus, 4 A. tesselatus. Of these dissected 1314 were A. maculatus, 27 A. hyrcanus, 29 A. minimus, 4 A. jeyporiensis, 1 A. tesselatus. No infections were found. The results of the precipitin reaction are given in Table V. Taking all the facts into consideration, it seems that A. maculatus does not carry malaria in this locality owing to its partiality for cattle blood.
Wong Chok Hang Village (Little Hong Kong).
38. Catching operations were continued throughout the year. The locality is surrounded by hills on all sides. A stream with several branches flows through it. A ravine which was formerly a rice swamp drains into the main stream. At the end of 1933 most of this swamp was ditched and divided into rectangular plots for growing crops such as Indian corn. During the present year no rice was grown. In surveys done at the beginning of the year, A. jeyporiensis larvae were found in good numbers in some of the ditches between the plots. The village people live in houses built of stone and roofed with tiles, the market gardeners in huts made of bamboo and roofed with thatch. All are engaged in growing crops and rearing pigs. The pigsties here are as a rule unsuitable as day-time resting places for Anophelines owing to their exposure to wind and weather. No cattle are kept in the locality, and dogs are now a scarce luxury on account of taxation.
39. From 8:30 a.m. until 11:30 a.m. collections were made by a catching coolie in two groups of huts on alternate days. These groups were situated north and south of Island Road, the northern group along the stream banks close to places A. minimus larvae had been found in abundance, the southern group about 440 yards downstream where few larvae of A. minimus had been found.
40. Of A. minimus, 2521 were obtained in 154 morning catches done in the northern group or 16.37 per morning. 792 were collected in 152 mornings from the southern group or 5.21 per morning.