are

52%

Keep the specimen suspended in the fluid so that all parts of it and freely Exposed Is the action of the fixative. The duration of fixation varies with the size of the blocke of tursue but in all cases it should be as shot as possible. Light need not be

foluded.

(6) Wash the block of tissue superficially

Kumming water.

in

(C) Suspend the specimen in rectified sporit

for several days. It would appear inmaterial,

within certain lunito, how long the lipue is left in spirit. I usually leave it for a considerable time, 29. as weet, to ten days. If a good Natural colour is NOT restored

within this time, it is advisab's, to shave off a thi, slice of the surface showing the Main pathological changes, and preserve it for a few days longer in as fresh spirit bath. In most cases it is advisable to add to this second bith a few grammes

of Potassium acetate and, Nitiate, sprinkling Ke the sails as carefully as possible over the

3-... surface.

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