505
Feeding guinea pigs with emulsions of plague cadavers, and cultures of the B. pestis, mixed with boiled ground rice, and dried over sulphuric acid. In the case of tissues, the organs or pieces selected for experiment were previously ground up in a mortar, and then well mixed with the rice in sterilised plate dishes. The cultures - both bouillon and agar-agar - were in every case 48 hours old and incubated at 36-37°C. They were poured over the rice and thoroughly mixed, with clean glass rods, through the rice, before placing the dishes before the animals. In order to put to the test the exact value of Klein's results with dried tissues and cultures, and small doses or amounts uniformly, culture and tissue were used uniformly throughout the experiments.
The details, as regards technique, are mainly similar to that already described under Series, No. I.
Table No. II. is a protocol of the main experiments.
Page 510
Page 511