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which has been added 1 to 1.5% of citrate of sodium.
After the blood has been added to the tubes it gradually settles as a deposit, leaving the superNatent broth clear in colour. The tubes are incubated at a temperature of 20 to 25°C. Should the superNatent fluid subsequently become cloudy, the tube is put aside as useless.
Kind or Kindo the may be
In addition to the inoculation of several tubes of the broth from each frog, I have always made several microscopic smear preparations from the blood, spleen, etc. in order to determine with some degree of accuracy the particular blood parasites present. Many frogs harbour Dactylosoma, Haemogregarina, and trypanosomata. Others show the parasites singly and this is borne out by the results of cultivation. Single species of parasites in frogs are rare, and involves the examination of a large number of animals.
The following results have been obtained by the use of these methods of cultivation :-
He...