166
TABLE XXI.
Characters of Organisms isolated from Samples or to 0145 inclusive.
504 organisms were isolated and worked out as shown in the following table. Four of the 504 were unclassed so that the table deals with 500.organisms.
Organisms from sample 01 to 0145 (inclusive). -
11 12 13 1.4 15 16
Group I Group II
Group III
Group IV
Media
1
2
3
4
5
6
7
8.
9 | 10 |
+
2
+
2
+*+*
++" |
tata f
+a +* |
+
+
I ut ut
2
+*+*
++" |
+~+~ |
+
+
Lactose
Litmus milk
Gelatine .......
Gram's stain
Motility.
Indol
+
6
Saccharose.
+
I
+
Days
2
Days
+~+~ |
+at+a |
{ ~+~+
| N+N+
+++ |
Į
+
+
2
10-14
I
+
+
+
+
+
+
+*+*
2
++*
1
* +
+
+
+
20+
+
16 +~+~
I
G
+
+
1
J
+
+
+
+
| + | 0
A
6
+
+a+a
+2 +2
+*+*
+*+*
+~+~
2
+
+
―
10-14 days
+
Tota
+a+a
6
+
+
+
= acid and gas, and Vosges and Proskauer's reaction. Indol formation or Motility as non-liquefaction of gelatine. Non-motile. the case may be.
No acid and gas.
+= a positive result.
+a+a
+2 +2
ta ta
+2 +2
2
2
I
ta ta
+2 +2
+++
No. of cultures "39" 8
-84 10-
9
107 15 15106 66 19.
1
9. 14. 1 7.
600.
Į
Į
+
2
N+ N+
+
2
Dulcit
Vosges and Proskauer's reaction
Glucose
LG
N. R. for-Fl\.]+
The Group distribution of the organisms isolated from the water when taken as a whole is practically the reverse of those isolated from human excreta, as will be seen from Table XXII.
TABLE XXII.
Total No. of cultures
407
1410
Sources of cultures
Chinese Coolies
Pokfulum Water
Group I......
32.4%
16.8%
Group II
41:7%
16.8%
Group III..........
19.7.%
36.0%
Group IV
3.2%
30.4%
Groups I and II in the water-isolated organisms are only half the percentage of the excretal isolated organisms, whereas in the water, Group III gives double the percentage and Group IV almost five times the percentage obtained from the Chinese coolies. If it is assumed that the reactions given by the various organisms isolated from the water are stable, the question arises, are all these different organisms of equal value as indicators of pollution?