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October 16th, 1904.-Scarification.
October 17th, 1904.-No result from vaccination. No rise in temperature.
October 18th, 1904.-Scarified again with the substance of the fresh spleen of no experimental pig. (Vide Experiment No. IV.—Pigs).
October 19th, 1904.-Scarification a failure so far. Animal apparently healthy. No rise in temperature. Blood examined microscopically with a negative result.. From this date onwards until November 1st, 1904, the animal remained in perfect healtb.
November 1st, 1904.-The animal was fed with 8 ounces of blood from a moribund case of acute Beri-beri.
November 3rd, 1904.-Fed again with 8 ounces of contents of flask used for Experiment No. IX. (Blood in Beri-beri).-Vide Bacteriological Experiments.
November 4th, 1904.--Again fed with 10 ounces of 96-hour growth of bouil- lon from flask used for Experiment No. IX. (Blood in Beri-beri).
November 8th, 1904.-Injected subcutaneously with 60 c.c. of the flask used for Experiment No. XI. (Blood in Beri-beri).
During this time when the animal was being subjected to so much experi- mentation the temperature remained normal, and the sheep fed well. There was no diarrhoea, no epigastric tenderness, and no difficulty in walking. The feeding experiments certainly brought any Beri-beri micro-organisms present in the blood into close relationship with the gastro-duodenal mucosa. Similarly the micro- organisins growing in the bouillon of the different flasks were incorporated in large quantity per the alimentary canal and the subcutaneous tissues.
From the 8th November onwards the sheep was kept under close obser- vation, and frequent tests were made to detect Beri-beri symptoms or sigus. Briefly it may be said that such signs were never found, and the animal maintained itself in good health for at least a year after the date of the last subcutaneous inoculation.
Observations on Calves.
Two calves were experimented with. Each was inoculated subcutaneously with the freshly drawn blood of an acute case of Beri-beri. Approximately 30 c.c. of blood was injected into each animal. Both animals lived for a week only, contracting hamorrhagic septicemia.
Owing to the great risks attached to the introduction of hæmorrhagic septicemia by cattle among our animals, it was considered advisable to stop further experiments with calves and coutine our attention to other animals.
Observations on Rabbits.
· A.—-NATURAL INFECTION,
One experiment of this nature was undertaken under similar conditions to those already detailed under "Natural Infection of Monkeys."
Four rabbits were placed in one of the rooms of the Po Leung Kuk, in which the inmates resided who contracted Beri-beri. The windows were shut and the light excluded as rigidly as possible. Previous to the cominencement of the experiment, the animals were kept under observation, and their blood tested microscopically.
The experiment lasted 6 months. The diet consisted of rice, vegetables, etc. At no time did they exhibit any sign of disease, and were as healthy whenr emoved as when they were first incarcerated.