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The animal was kept under close observation. The temperature was noted twice daily, but never deviated from normal.
The animal moved and functionated normally, took its food well, had no diarrhoea or epigastric tenderness and no cedema was ever present.
Five months after the date of the last injection the animal was well. There was not the slightest sign of Beri-beri. The knee-jerks were normal.
The animal then contracted dysentery and died a few days after.
Experiment No. II.
Animals-One monkey.
The animal was normal previous to the commencement of the experiment, and the usual precautions were taken.
October 27th, 1904.-10 c.c. of freshly drawn venous blood from an acute case of Beri-beri was inoculated.
October 21st, 1904.-8 c.c. of blood freshly drawn from an oedematous case was injected. No change was noted in the general behaviour of the animal. The temperature was always normal. The knee-jerks were frequently tested but were always present and not exaggerated.
The period of observation of this animal extended over one year. At the end of this time the condition of the animal was that of a normal monkey. Beri-beri- like symptoms or signs never manifested themselves.
E-FEEDING ExperimentS WITH GASTRIC MUCOUS MEMBRANE TAKEN FROM
FRESH AND FATAL CASES OF ACUTE BERI-BERI.
Experiment No. I,
Animal-One monkey.
Healthy, fed well and moved like other monkeys.
November 12th, 1904.-The animal was fed with a mixture of boiled rice and broken down gastric mucosa obtained an hour after death from an acute case of Beri-beri. The pieces of mucosa used for the experiment contained small erosions and hæmorrhages, presumably the pathological changes described by WRIGHT, and insisted on by him as the primary lesion in Beri-beri.
The bolus was coaxed down the animal's throat. No sickness followed and the animal was carefully observed until the following day, lest it might vomit the mixture. Nothing followed however, and on the next morning the monkey appeared to be in good health. The temperature was normal.
From November 13th, 1904, until March, 1905, the monkey was kept under close watch. It never shewed any sign of Beri-beri. It moved, climbed, ate, and behaved generally like any other monkey. The result therefore was negative, although the bolus administered contained presumably WRIGHT's primary lesion and his diphtheriod bacillus and toxin, and was brought into actual contact with the gastro duodenal mucosa of the monkey.
Experiment No. II.
Animal-One monkey.
This was fed in exactly the same way and with the same ma-
terial, including pieces of the duodenum.
Result-Negative as regards Beri-beri after four months' observation.