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From this time onwards the animal kept in perfect health. It functionated normally and all its motor and sensory apparatus responded normally to the usual stimuli. There was never any epigastric tenderness, and the knee-jerks could be daily elicited with ease.
At the present time-one year after the commencement of the experiment— the animal is alive and quite lively.
(3) With Cerebro-Spinal Fluid.
Animal-One monkey.
After observing the monkey for several days 5 c.c. of freshly drawn cere- bro-spinal fluid from a marked case of oedematous Beri-beri was injected subcutaneously, on October 4th, 1904, and the animal was subsequently examined daily.
October 19th, 1904.-Still healthy and functionates normally. The injection of the fluid has practically caused the animal no inconvenience.
October 20th, 1904.-Was injected subcutaneously with 10 c.c. venous blood of a recent and acute case of Beri-beri. This injection caused no elevation of temperature, and the animal functionated like other monkeys.
but
October 26th, 1904.-There is a suspicion of weakness in the bind limbs, a positive opinion on this matter is difficult to give as the animal appeared dull and apathetic. The knee-jerks were exaggerated however, and the animal resented pressure on its epigastrium. There was no evidence of disturbance of sensation. There was no edema and no muscular tenderness.
October 7th, 1904.-The animal received another injection of 8 c.c. of blood from an acute case of Beri-beri.
November 1st, 1904.-The last injection caused no inconvenience to the animal. There was no temperature and the animal took its food and functionated normally. On careful examination the weakness of the hind legs is apparently lost. The animal could climb, grasp firmly, and behave on its legs like other monkeys. There was no change in the knee-jerks, and no epigastric discomfort on gentle pressure. The monkey in fact appears to be in perfect health.
November 8th, 1904.-Diarrhea set in to-day. This is mucoid and bloody in character. No fever.
November 10th, 1904.-The animal died to-day, the result of persistent diarrhoea. It exhibited no signs of Beri-beri infection up to the date of its death.
Post-mortem Examination-Mucous colitis. Other organs normal. No signs of Beri-beri, and no evidence of a gastro duodenitis (localised) as described by HAMILTON WRIGHT. Microscopical examination of smears of the various organs gave a negative result, and the bacteriological examination of the blood and the spleen resulted in nothing of a definite nature being found.
D.--INTRA-PERITONEAL INJECTION OF FRESHLY DRAWN BLOOD.
Experiment No. I.
Animal-One monkey.
Preliminary precautions taken as before.
October 27th, 1904.-4 c.c. of blood from an acute cedematous case of Beri- beri injected intraperitoneally.
October 31st, 1904.-6 c.c. blood again injected into the peritoneum from another acute case of Beri-beri.