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A temperature chart is not appended as the course of the fever when pre- sent was really the same as in the other experiments. At no time while the ani- mal was under these observations did it shew any change from its normal condition. After being kept in its cage for 6 months it escaped and was lost.
Experiment No. VI.
Animal-One monkey.
Nature of experiment :—
The same as the previous ones and under the same conditions.
Forty e.c. of blood from four different recurrent cases of Peri-
beri were injected.
The animal lived for 3 months and was healthy. It contracted dysentery and died. Post-mortem, there was no evidence of Beri-beri, no oedema, no changes in stomach and duodenum.
Experiment No. VII.
Animal-One monkey.
Nature of experiment :-
As before.
Three injections of blood 33 c.c. were given and the animal is alive and well after one year.
(2) With Beri-beri Spleen Blood.
Experiment No. 1.
Animal-One monkey.
Nature of experiment :—
The animal was kept under observation for several days prior to commencing the experiment. A case of acute Beri-beri with enlarged spleen was selected and blood films prepared for examination. No malarial parasites or other organisms were found. The case containing the monkey was brought along the bedside, and splenic puncture performed under aseptic conditions. The bloody fluid obtained was at once injected subcutaneously into the animal in all about 8 cc. was used. This inoculation was made on September, 27th, 1904. A drop of this fluid was spread on a micros- cope slide, stained and examined. Nothing was found. microscopically. Bacteriologically this fluid gave a negative result,
The attached temperature chart shews that the injection was followed by a slight degree of fever with gradual defervescence, and lasting 5 to 6 days. This transitory temperature can be reasonably ascribed to the result of the injection. There was no evidence that it had anything to do with the onset of Beri-beri. A day or two after the inoculation the blood of the animal was examined but was negative as regards germs. No change in the physical appearance of the monkey was observed after the injection.
On the 11th October, namely, fourteen days after the inoculation of splenic blood, the animal was fed according to the methods already described, with 20 c.c. of freshly drawn blood of a dropsical case of Beri-beri. This again occasioned an evanescent rise in temperature lasting 3-4 days. Subsequent to this the temperature became normal, so that a prolongation of the curve on the attached chart was unnecessary.