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After a mouth's observation of the monkey it was fed with an emulsion of fresh spleen in normal sterile saline, obtained post-mortem from a Beri-beri cadaver of a few hours old. The temperature was taken twice daily as before, and the physical condition of the animal noted weekly. The temperature chart is not recorded for this experiment. it practically runs the same course as that recorded for Experiment No. 1.
The experiment was commenced on 11th October, 1904, the temperature and the physical conditions of the animal were recorded until the 27th January, 1905, i.c., for 3 months.
At the present time (October, 1905), i.e., one year since the commencement of the experiment the animal is alive and healthy.
C-SUBCUTANEOUS INOCULATION.
(1) With Beri-beri blood.
Experiment No. I.
Animal-One monkey.
Nature of experiment :-
The animal was inoculated subcutaneously with 5 c.c. blood obtained, under sterile conditions, from the median basilic vein of a patient suffering from acute Beri-beri. The monkey was kept under observation for a few days previous to the coininencement of the experiment, its temperature and physical condition being noted. When the experiment was made, the monkey was brought to the bedside of the patient and the blood transferred directly from the arm of the patient to the monkey by means of a sterilised syringe.
The inoculation was made on the 27th September, 1904. 5 c.c. of blood was injected into the subcutaneous tissue over the abdomen.
The attached tempera- ture chart shews the course of the body heat during the period of observation. The blood of the animal was examined previous to the commencement of the ex- periment. It shewed nothing abnormal.
The injection of the blood caused a slight rise in temperature which continued for about 8 or 9 days. During this period the animal remained in perfect health, eating well, with no diarrhoea, and all sensory and motor phenomena were carried out and responded to in a normal fashion. The blood was negative as regards organisms.
After observing the animal for a fortnight a feeding experiment was performed. On the 11th October the animal was fed with 30 c.c. of freshly drawn blood from an acute case of Beri-beri. The technique employed was the same as that des- cribed under Feeding Experiments.
The blood
This caused a transient rise in temperature, but notwithstanding, the animal fed well, and moved and responded to all stimuli in a normal manner. was again examined but with a negative result.
After another forthnight had passed, the monkey received another subcutane- ous injection of blood, nearly 5 c.c. taken as before from an acute ædematous case of Beri-beri.
This was followed by a rise in temperature lasting 3-4 days. During this period-a month after the first injection-the monkey remained in perfect health.
From this time onwards the temperature remained normal and for this reason the curve has not been continued on the chart.
During the middle of December, 1904, the animal contracted dysentery and died on the 26th December.