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6.-EXPERIMENTAL
RESEARCHES.
These researches occupied the greater part of our time and seemed to offer us the best opportunity of locating the exact etiological factor in Beri-beri. After the account given by HAMILTON WRIGHT of natural Beri-beri infection of monkeys, coupled with the negative results obtained by other methods of investigation, it appeared to us experimental methods would in all probability bring to light this much sought for factor in Beri-beri. Naturally these experiments were undertaken from as wide a standpoint as possible. Monkeys were used on a large scale and subjected to experimentation by almost every method known. In addition, pigs, sheep, calves and a horse, rabbits and fowls were made extensive use of. The following is a resumé of the inethods adopted, with a detailed account. of the experiments themselves:-
C
1.-Observations on Monkeys.
A.-Natural Infection.
B.-Feeding experiments-
(1) With Beri-beri blood.
(2) With spleen substance.
(3) With gastro duodenal mucosa.
C-Subcuntaneous Inoculation-
(1) With Beri-beri blood.
(2) With spleen blood.
(3) With cerebro-spinal fluid.
D.-Intraperitoneal inoculation with Beri-beri blood.
2. Observations on Pigs.
4.-Feeding experiments-
(1) With Beri-beri blood. (2) With spleen substance.
(3) With brain substance.
(4) With cerebro-spinal fluid.
(5) With gastro duodenal mucosa and other abdominal organs.
3. Observations on Sheep.
A-Vaccination experiments with spleen pulp.
B-Feeding experiments with Beri-beri blood.
4.-Observations on Calves.
Subcutaneous inoculation with Beri-beri blood.
5. Observations on Rabbits.
A.-Natural infection.
B. Subcutaneous inoculation with Beri-beri blood.
C.-Feeding experiments-
(1) With Beri-beri blood.
(2) With spleen substance.
6. Observations on Fowls.
A.-Feeding experiments with spleen substance and blood.
7.-Observations on a Horse.
4.-Subcutaneous inoculation with Beri-beri blood.