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November 9th, 1904.-3 c.c. from the same flask was again incorporated subcutaneously.
November 10th, 1904. -No reaction found except a small localised swelling. The temperature became subnormal.
November 11th, 1904.-10 c.c. of the contents of the flask used for Experiment No. X was injected subcutaneously.
November 12th, 1904.-Local reaction found but no elevation of temperature, November 13th, 1904.-Diarrhea set in.
November 14th, 1904.-The animal died to-day.
Throughout the experiments, no signs of symptoms of Beri-beri shewed themselves. The doses of the culture given were large but it must be remembered that previous experiments had shewn the culture to be possessed of but small virulence.
Post-mortem Examination.—The condition of the internal organs was similar to that found in Experiment No. I, and need not be detailed.
The cause of death was chronic poisoning due to the incorporation of large amounts of staphylococci and their ptomaines.
One Rabbit.
Experiment No. X C.
After determining that the animal was in perfect health, the following ex- periment was made.
November 7th, 1904.-2 c.c. of the bouillon growth was injected sub- cutaneously.
November 8th, 1904.-The temperature is 104° F. The animal is dull and apathetic, and refuses food.
November 9th, 1904.-The temperature was normal. The animal was livelier to-day. The swelling at the site of inoculation is fully developed but shews no signs of softening. The animal was tested for the presence of signs of Beri-beri. The knee-jerks were present. There was no epigastric tenderness and no tender- ness on pressure over the muscles.
November 10th, 1904.-2 c.c. of the same bouillon again injected beneath the skin of the abdomen?
November 13th, 1904.--Still no signs of Beri-beri.
November 15th, 1904.–2 c.c. again injected.
November 18th, 1904.-No evidence of Beri-beri.
November 21st, 1904.-2 c.z. again injected.
November 25th, 1904.-There is no evidence of a sign of Beri-beri. The animal moved and functionated like other normal rabbits.
November 29th, 1904.-Diarrhoea set in.
December 2nd, 1904.-The animal died to-day.
Post-mortem Examination.-Cause of death was diarrhoea. Absolutely no sign of Beri-beri.
Experiment No. XI.
The conditions and technique of the experiment were the same as in the pre- vious experiments. A well marked case of olematous Beri-beri was chosen for the venesection.
The flask was subsequently incubated at 37° C.