165
Experiment No. X.
The nature of this experiment and the technique employed were identical with that found in Experiments Nos. VIII and IX.
After incubation of the flask at 37° C. for 48 hours, a mixed growth of cocci were found. These proved to be staphylococci, chromogenic and non-chromoge- nie varieties being both present. There was nothing distinctive about these mi- cro-organisms. They answered to all the characteristics and cultural tests of the ordinary ubiquitous staphylococci.
The contents of this flask were utilised for the carrying out of the following experiments-
One Monkey.
Experiment No. X A.
The animal was previously tested as to its good health and during the experiment the temperature was tested twice daily.
November 7th, 1904.--The animal received subcutaneously 10 c.c. of the cul- ture in the flask.
November 8th, 1904.-The inoculation made yesterday appeared to have no effect upon the animal. There was a slight local swelling over the site of the ino- culation. There was however no fever.
Thereupon, 10 c.c. of the flask used for Experiment No. XI was injected subcutaneously.
November 11th, 1904.-Diarrhoea set in to-day. The animal appears weak and very apathetic. It can only with difficulty be made to stir from the crouching position.
November 12th, 1904. The diarrhoea has become more pronounced. The animal appears to be in a condition of septic poisoning. The knee-jerks are pre- sent and the monkey shrieks when touched. It is evidently in great pain. There are no evidences present of acute Beri-beri.
November 14th, 1904.—The animal died to-day.
Post-mortem Examination.-The body was greatly emaciated. Rigor mortis was present (4 hours after death). The subcutaneous tissues shewed no cedema or mucoid appearance. The lungs were normal. There was no excess of fluid in the pleural cavities. The heart was dilated and contained dark fluid blood. Several small ante-mortem clots were found present. The tardiac muscle was found to be soft and flabby and degenerated. There was no endocarditis. The mucous membrane of the stomach was injected and eroded, with numerous patches. of hæmorrhagic extravasation. The intestine throughout was in a condition of acute follicular enteritis. The mesenteric glands were enlarged and soft and shewed points of hæmorrhagic extravasations. The spleen, apart from congestion, was norinal. The kidneys were congested. Smear preparations were made from the spleen and the heart blood, and shewed the presence of staphylococci. This was confirmed bacteriologically. The cause of death was put down as staphylo- coccic ptomaine poisoning.
One Monkey.
Experiment No. X B.
The conditions were the same as those of Experiment No. I. The usual preliminary precautions were taken.
November 7th, 1904.---The monkey was inoculated subcutaneously with 6 c.c. of the culture in the fluid.
November 8th, 1904.-A small localised swelling was found over the site of the inoculation. There was no rise in temperature.