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Experiment No. IX A.
One rabbit was inoculated subcutaneously on November 3rd, 1904, with the bouillon containing both varieties of organisms.
On the following day a small localised swelling was found over the seat of inoculation. The temperature was normal.
The animal continued in a good state of health until November 8th, 1904, when it received 2 c.c. of a 24-hr. old bouillon culture of the staphylococci present in this flask.
During the next few days the rabbit remained well.
November 12th, 1904.-Another 2 c.c. of the bouillon culture of the micro-cocci were inoculated subcutaneously.
November 18th, 1904.-2 c.c. injected as on November 12th.
November 20th, 1904-The animal is well, feeds, and has no diarrhoea. A small abscess has developed over the site of the injection of November 12th. The knee-jerks are present, there is no apparent terderness on pressure over the abdomen, and the rabbit moves and functionates as other animals do.
November 25th, 1904.-2 c.c. of the flask used for Experiment No. XI were injected subcutaneously.
From this time onwards until the middle of January, 1905, the rabbit remained in a fair state of health. There was never any sign of Beri-beri. About the latter end of January subcutaneous abscess formation took place and the animal died from general septic poisoning.
Although the animal was frequently examined during the experiment, which lasted about three months, no sign of Beri-beri was elicited. There was never any distension of the abdomen or epigastric tenderness on gentle pressure.
The knee-jerks could always be elicited and pressure on the leg muscles did not appear to evoke any discomfort.
Experiment No. IX B.
The experiment is similar in almost all respects to the previous one.
November 3rd, 1904.-One rabbit was inoculated subcutaneously with 3 c.c. of the bouillon.
November 8th, 1904-5 c.c. of the contents of the flask used for Experiment No. XI, inoculated subcutaneously.
The result of these injections was negative. Apart from localised swellings over the site of inoculation, the animal shewed no discomfort. The temperature remained normal. It will be obvious from these inoculations that the micro- organisms present in the flask possess practically no pathogenicity.
November 19th, 1904.-The animal contracted pneumonia and died the follow- ing day.
There never was any sign of paralyses, epigastric tenderness, cedema, or tenderness of the muscles. The knee-jerks could always be elicited.
Post-mortom Examinatien.-Double fibrinous pneumoina; no evidence of lesions indicative of Beri-beri found.
Experiment No. IX C.
For details of this Experiment reference should be made to Observations on Sheep-Experiment No. 2.