Sessional_Paper_1906 — Page 309

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Observations on Fowls.

Experiment No. I.

One fowl.

The animal was kept under observation for a week previous to the commen- cement of the experiment. Its temperature was normal varying from 105°-106° F. November 1st, 1904.-Fed with broken down spleen substance obtained from a cadaver with typical acute Beri-beri lesions.

November 8th, 1904.-Fed with 30-40 c.c. of the contents of the flask used for Experiment No. XI (vide "Organisms in Blood of Beri-beries ").

From this date onwards, the animal shewed no departure from its normal state of health. A temperature chart is not appended as no fever was ever present. The bird was kept under observation for at least six months from the last date of feed- ing and at no time shewed any symptom or sign of Beri-beri,

Experiment No. II.

Five fowls.

Kept in a lien coop

isolated.

Normal previous to commencement of experiment.

October 25th, 1904.– Fed with broken down spleen of a recently dead acute case of Beri beri. The spleen was enlarged and weighed 1 lbs. The fowls devoured the whole organ.

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November 1st, 1904. Fed with a second spleen under similar conditions. This was also actively devoured.

The birds were kept under close observation and tested almost daily as regards their powers of locomotion. Their temperature never appeared to vary, and at no time during the following six months was there the slightest evidence of Beri-beri infection.

Experiment No. III.

One hen.

November 1st, 1904.-Fed with the broken down spleen pulp of a recently dead case of acute Beri-beri.

November 8th, 1904.-Fed with an emulsion of boiled rice and 100 c.c. of contents of flask used for Experiment No. XI (vide "Blood in Beri-beri.”)

The animal was kept under observation for at least three months. The temperature was noted daily. It continued to move and functionate like any other normal fowl. Up to the date of the last observation, it never shewed the slightest trace of Beri-beri.

Observations on a Horse.

A horse, China pony, was placed under observation and its temperature and general health noted for some days.

As

October 25th, 1904.-Inoculated subcutaneously (over left side of neck) with 60. c.c. of defibrinated fresh venous blood taken from a case of acute Beri-beri. the result of the injection a hard brawny swelling occurred around the site of inoculation. This gradually subsided and was entirely gone in about 3 weeks. It occasioned no rise in the temperature of the animal and apparently gave him no discomfort. The animal fed well, had no diarrhea, and moved and function- ated like a normal horse.

October 27th, 1904.-The animal was again inoculated subcutaneously with with 80 c.c. of freshly drawn venous blood from a patient suffering from acute dedematous Beri-beri. This occasioned another hard swelling about the size of a child's head which gradually subsided.