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Experiment No. 4.-2 bugs completely immersed for 15 minutes.
After removal from the solution, the animals appeared to be dead and
remained in that condition for about 20 minutes.
Complete recovery of both animals took place in about 45 minutes after
removal from the solution.
In connection with this (A. Series of Experiments) a few interesting phenomena were noted. Immediately on immersion, the animals shewed vigorous movements of the legs and moved about actively in the fluid. This, in a few seconds, is succeeded by a few convulsive efforts in all the extremities continuing from 1 to 2 seconds. Subsequently all movements ceased.
On expiration of the time limit of the particular experiment, the animals were transferred to a clean, dry glass capsule and frequently examined by a low power miscroscopic objective. For some considerable time no movements on the part of the animal were detected, e.g., Experi- ment No. 1.—For 5 minutes. At the expiration of this time slight convulsive seizures in the body and extremities commenced; these became gradually more pronounced and eventually more purposive until the animals completely recovered.
Washing the animals in water after immersion in Jeyes' Fluid solution or
exposure to the sun accelerates their recovery.
B.-SERIES.
2 Parts or 2 c.c. of Jeyes' Fluid in 100 c.c. of water.
Experiment No. 1.—2 bugs completely immersed for 1 minute.
After removal, the animals appeared to be dead. Movements of the ex-
tremities cominenced in about 10 minutes.
Both animals completely recovered in 20 minutes.
Experiment No. 2.—2 bugs completely immersed for 5 minutes.
After removal, animals remained motionless.
Muscular movements commenced in 15 minutes.
Both animals recovered in 30 minutes.
Experiment No. 3.-2 bugs completely immersed for 15 minutes.
After removal of the animals, the same phenomena were observed. Muscular movements commenced in 15 minutes.
Both animals completely recovered in 35 minutes.
C.-SERIES.
3 Parts or 3 c.c. Jeyes' Fluid in 100 c.c. of water.
Experiment No. 1.-2 bugs completely immersed for 1 minute.
On removal, same phenomena as before.
Muscular movements began in 15 minutes.
Complete recovery of both animals in 45 minutes.
Experiment No. 2.-2 bugs completely immersed for 5 minutes.
On removal, same appearances met with as in previous experiments. Muscular movements began in 30 minutes, in one animal. This bug
was quite lively after 1 hour.
The other animal was dead,
Experiment No. 3.—2 bugs completely immersed for 5 minutes.
Both animals were dead on removal from the solution.
The observation extended over 4 hours.
This series of experiments was repeated with almost similar results.
D. SERIES.
4 Parts or 4 c.c. of Jeyes' Fluid in 100 c.c. of water.
Experiment No. 1.-2 bugs completly immersed for 1 minute.
After removal, the same phenomena were observed as before. Both animals were quite lively in 10 minutes.
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