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Y.H. CHEUNG, K.Y. TAI, S.W. TSAO AND L.B. THROWER
soluble carbohydrates by the anthrone method.
e) Colonization of submerged Kandelia leaves
Colonization by fungi and bacteria was investigated. Matched sets of senescent leaves were immersed in plastic mesh bags (1 × 1 mm, mesh). Individual bags were collected at intervals and processed in the laboratory. For isolation of fungi the leaves were washed thoroughly with 7 changes of 1% detergent solution and 8 changes of sterile water. Each leaf was cut in half longitudinally: one half was plated on to Czapek-Dox agar for surface fungi, while the other half was surface-sterilized with 0.1 M mercuric chloride solution, washed in sterile water and plated on to Czapek-Dox agar for isolation of fungi present within the tissue. The plates were incubated at 25-27°C, and representative colonies were isolated and counted.
A sterile swab was used to remove the surface film of bacteria from the leaves, and this was streaked on to plates of nutrient agar or marine agar 2216. After incubation at 25-27°C, representative colonies were isolated and characterized by their Gram staining and various physiological properties.
f) Diet of Higher Trophic Levels
This was determined by digestive tract analysis on a range of animals. Contents of the stomach (fish), buccal cavity (shrimps) or gut (amphipods, insect larvae, worms) were removed and examined with the microscope to estimate the fractions of various materials.
g) Effect of Birds on Productivity
Birds are the only carnivorous animals that may compete with man for the economic produce of the kei wais. Consequently, an estimate was made of the amount of produce removed by them. Information on the diet of birds at Mai Po was obtained from Melville (1978). Hulscher (1975) has suggested that the energy intake of birds is about five times the basal metabolic rate, while King and Farmer (1961) have proposed the following formula for
190
Y.H. CHEUNG, K.Y. TAI, S.W. TSAO AND L.B. THROWER
soluble carbohydrates by the anthrone method.
e) Colonization of submerged Kandelia leaves
Colonization by fungi and bacteria was investigated. Matched sets of senescent leaves were immersed in plastic mesh bags (1 ×1 mm, mesh). Individual bags were collected at intervals and pro- cessed in the laboratory. For isolation of fungi the leaves were washed thoroughly with 7 changes of 1% detergent solution and 8 changes of sterile water. Each leaf was cut in half longitudinally: one half was plated on to Czapek-Dox agar for surface fungi, while the other half was surface-sterilized with 0.1 M mercuric chloride solution, washed in sterile water and plated on to Czapek-Dox agar for isolation of fungi present within the tissue. The plates were incubated at 25-27°C, and representative colonies were iso- lated and counted.
A sterile swab was used to remove the surface film of bacteria from the leaves, and this was streaked on to plates of nutrient agar or marine agar 2216. After incubation at 25-27°C, representative colonies were isolated and characterized by their Gram staining and various physiological properties.
f) Diet of Higher Trophic Levels
This was determined by digestive tract analysis on a range of animals. Contents of the stomach (fish), buccal cavity (shrimps) or gut (amphipods, insect larvae, worms) were removed and ex- amined with the microscope to estimate the fractions of various materials.
g) Effect of Birds on Productivity
Birds are the only carnivorous animals that may compete with man for the economic produce of the kei wais. Consequently, an estimate was made of the amount of produce removed by them. Information on the diet of birds at Mai Po was obtained from Melville (1978). Hulscher (1975) has suggested that the energy intake of birds is about five times the basal metabolic rate, while King and Farmer (1961) have proposed the following formula for
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