M 60
(6) Preparation of Vaccine Lymph.
313. In the Report of last year reference to the preparation of lymph was made to the effect that this was "much accelerated"; it will be seen that the large amount referred to in 1937 has been more than doubled in the year under review. The amount issued is also more than double that of the previous year.
314. The output of this large amount of lymph involved the issue of freshly prepared or "green" lymph, and this in turn required some special method of treatment in order that the bacterial content should be within the limits imposed by the "Therapeutic Substances Act". Fortunately, some experimental work on this point had been in progress for the past two years at the Institute and we were able to put our results to practical use at a time when they were badly needed.
315. The method used is briefly as follows:
The preparation of the lymph is carried to completion in the usual manner. Anaesthetic ether, 2 per centum by volume, is then added, and the container vigorously shaken so as to mix the contents intimately. The lymph is then stored at refrigerator temperature for a period of not less than twelve days or more than two weeks. At the expiration of this time bacteriological and potency tests are carried out as usual. If these tests prove satisfactory, air is bubbled through the lymph for about twenty minutes in order to remove the ether; the lymph is then ready for use.
316. The bacterial colony count will be found invariably to be well within the prescribed limits and the potency quite unimpaired. By the use of this method of purification we were enabled on occasions to issue lymph no older than three weeks with perfectly satisfactory results.
Amount of lymph prepared 59,562 c.c. issued 45,194 c.c. in stock at end of year 32,845 c.c. No. of buffalo calves scraped for pulp 65 Total pulp collected 13,040 g. Average per buffalo calf 200.6 g. No. of cow calves scraped 1 No. of sheep scraped 15 Total pulp collected 241.0 g. Average per sheep or cow calf 40.16 g.317. The small yield obtained from cow-calves and sheep is a point worthy of note. The average yield per buffalo-calf, on the other hand, is a record for this Institute. All other figures given also exceed any previously reported; and it should be emphasized that this enormous quantity of lymph was made and distributed by the same staff and equipment as in previous years.
(7) Preparation of Vaccines and Sera.
318. Antimeningococcus serum.-The issue this year was exceptionally large, 48,780 c.c.; an amount actually in excess of the quantity held in stock at the end of 1937. The amount prepared was 17,900 c.c. It is obvious that the supply will have to be augmented by purchase during the coming year. It is impossible to produce more than we are doing from three ponies and keep them in good health under the unsuitable conditions in which they live.
M 60
(6) Preparation of Vaccine Lymph.
313. In the Report of last year reference to the preparation of lymph was made to the effect that this was "much accelerated"; it will be seen that the large amount referred to in 1937 has been more than doubled in the year under review. The amount issued is also more than double that of the previous year.
314. The output of this large amount of lymph involved the issue of freshly prepared or "green" lymph, and this in turn required some special method of treatment in order that the bacterial content should be within the limits im-
posed by the "Therapeutic Substances Act”. Fortunately, some experimental work on this point had been in progress for the past two years at the Institute and we were able to put our results to practical use at a time when they were badly needed.
315. The method used is briefly as follows:
manner.
The preparation of the lymph is carried to completion in the usual Anaesthetic ether, 2 per centum by volume, is then added, and the container vigorously shaken so as to mix the contents intimately. The lymph is then stored at refrigerator temperature for a period of not less than twelve days or more than two weeks. At the expiration of this time bacteriological and potency tests are carried out as usual. If these tests prove satisfactory, air is bubbled through the lymph for about twenty minutes in order to remove the ether; the lymph is then ready for use.
316. The bacterial colony count will be found invariably to be well within the prescribed limits and the potency quite unimpaired. By the use of this method of purification we were enabled on occasions to issue lymph no older than three weeks with perfectly satisfactory results.
Amount of lymph prepared
Table XLI.
59,562 c.c.
issued
45,194 c.c.
JJ
in stock at end of year
32,845 c.c.
"
No. of buffalo calves scraped for pulp
65
Total pulp collected
13,040 g.
Average per buffalo calf
No. of cow calves scraped
200.6 g.
1
15
sheep scraped
Total pulp collected
5
241.0 g.
Average per sheep or cow calf
40.16 g.
317. The small yield obtained from cow-calves and sheep is a point worthy of note. The average yield per buffalo-calf, on the other hand, is a record for this Institute. All other figures given also exceed any previously reported; and it should be emphasized that this enormous quantity of lymph was made and distributed by the same staff and equipment as in previous years.
(7) Preparation of Vaccines and Sera.
318. Antimeningococcus serum.-The issue this year was exceptionally large, 48,780 c.c.; an amount actually in excess of the quantity held in stock at the end of 1937. The amount prepared was 17,900 c.c. It is obvious that the supply will have to be augmented by purchase during the coming year. It is impossible to produce more than we are doing from three ponies and keep them in good health under the unsuitable conditions in which they live.
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