AnnualReport-1933 — Page 487

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M 123

(4) Publications :

by

"Perforative Amoebic Ulceration of the Appendix,

A. V. Greaves, Transactions of The Royal Society of Tropical Medicine and Hygiene, Vol. XXVI, No. 4, January, 1933.

"Acute Phlegmon of the Stomach and Duodenum," by A. V. Greaves, Canadian Medical Association Journal, Vol. XXIX, 37-39, 1933.

The Laboratory Diagnosis of Malaria," by A. V. Greaves,

The Caduceus, Vol. XII, No. 4, November, 1933. "A New Microscope Adapter for the Hand Spectroscope,"

by A. V. Greaves, (In the Press).

(5) Research—Routine work in great volume again prevented much being done in the way of research.

(a) Dysentery.—The work on Flexner dysentery strains commenced during the latter part of 1932 has been pursued steadily and although the number of cultures isolated and dealt with is not large, some facts emerging are of considerable interest.

The appended table shows the distribution of the strains between the various sub-divisions of the group and those which are inagglutinable.

VWXYZZX 7%28%13%7%2%28% 9%6%MixedInagglutinable

The infrequence of inagglutinable strains is somewhat remarkable in view of the records of Indian observers. Thus Manifold and deMonte report 28.2% of 117 strains as inagglutinable. It is possible that the reason for this may be in the fact that in the latter group agglutination was carried out relatively soon after isolation, whereas in our series all strains isolated were subcultured weekly for three months before agglutination, thus permitting any agglutinable property to become manifest and fixed. The type sera used were those of Medical Research Council and all the strains recorded showed agglutination to 50% or over titre. Boyd's experience regarding saccharose fermentation has been confirmed by us, no agglutinable culture showing true saccharose fermentation. One of the inagglutinable cultures, however, showed definite acidity in saccharose. This strain has not yet been investigated thoroughly.

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M 123 (4) Publications : by "Perforative Amoebic Ulceration of the Appendix, A. V. Greaves, Transactions of The Royal Society of Tropical Medicine and Hygiene, Vol. XXVI, No. 4, January, 1933. "Acute Phlegmon of the Stomach and Duodenum," by A. V. Greaves, Canadian Medical Association Journal, Vol. XXIX, 37-39, 1933. The Laboratory Diagnosis of Malaria," by A. V. Greaves, The Caduceus, Vol. XII, No. 4, November, 1933. "A New Microscope Adapter for the Hand Spectroscope," by A. V. Greaves, (In the Press). (5) Research—Routine work in great volume again prevented much being done in the way of research. (a) Dysentery.—The work on Flexner dysentery strains commenced during the latter part of 1932 has been pursued steadily and although the number of cultures isolated and dealt with is not large, some facts emerging are of considerable interest. The appended table shows the distribution of the strains between the various sub-divisions of the group and those which are inagglutinable. VWXYZZX 7%28%13%7%2%28% 9%6%MixedInagglutinable The infrequence of inagglutinable strains is somewhat remarkable in view of the records of Indian observers. Thus Manifold and deMonte report 28.2% of 117 strains as inagglutinable. It is possible that the reason for this may be in the fact that in the latter group agglutination was carried out relatively soon after isolation, whereas in our series all strains isolated were subcultured weekly for three months before agglutination, thus permitting any agglutinable property to become manifest and fixed. The type sera used were those of Medical Research Council and all the strains recorded showed agglutination to 50% or over titre. Boyd's experience regarding saccharose fermentation has been confirmed by us, no agglutinable culture showing true saccharose fermentation. One of the inagglutinable cultures, however, showed definite acidity in saccharose. This strain has not yet been investigated thoroughly.
Baseline (Original)
M 123 (4) Publications : > by "Perforative Amoebic Ulceration of the Appendix, A. V. Greaves, Transactions of The Royal Society of Tropical Medicine and Hygiene, Vol. XXVI, No. 4, January, 1933. "Acute Phlegmon of the Stomach and Duodenum," by A. V. Greaves, Canadian Medical Association Journal, Vol. XXIX, 37-39, 1933. The Laboratory Diagnosis of Malaria," by A. V. Greaves, The Caduceus, Vol. XII, No. 4, November, 1933. "A New Microscope Adapter for the Hand Spectroscope," by A. V. Greaves, (In the Press). (5) Research-Routine work in great volume again prevent- ed much being done in the way of research. (a) Dysentery.—The work on Flexner dysentery strains commenced during the latter part of 1932 has been pursued steadily and although the number of cultures isolated and dealt with is not large, some facts emerging are of considerable in- terest. The appended table shows the distribution of the strains between the various sub-divisions of the group and those which are inagglutinable. V W X Y Z ZX 7% 28% 13% 7% 2% 28% 9% 6% Mixed Inagglutinable The infrequence of inagglutinable strains is somewhat re- markable in view of the records of Indian observers. Thus Manifold and deMonte report 28.2% of 117 strains as inagglutin- able. It is possible that the reason for this may be in the fact that in the latter group agglutination was carried out relatively soon after isolation, whereas in our series all strains isolated were subcultured weekly for three months before agglutination, thus permitting any agglutinable property to become manifest and fixed. The type sera used were those of Medical Research Council and all the strains recorded showed agglutination to 50% or over titre. Boyd's experience regarding saccharose fer- mentation has been confirmed by us, no agglutinable culture showing true saccharose fermentation. One of the inagglutin- able cultures, however, showed definite acidity in saccharose. This strain has not yet been investigated thoroughly.
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M 123

(4) Publications :

>

by

"Perforative Amoebic Ulceration of the Appendix,

A. V. Greaves, Transactions of The Royal Society of Tropical Medicine and Hygiene, Vol. XXVI, No. 4, January, 1933.

"Acute Phlegmon of the Stomach and Duodenum," by A. V. Greaves, Canadian Medical Association Journal, Vol. XXIX, 37-39, 1933.

The Laboratory Diagnosis of Malaria," by A. V. Greaves,

The Caduceus, Vol. XII, No. 4, November, 1933. "A New Microscope Adapter for the Hand Spectroscope,"

by A. V. Greaves, (In the Press).

(5) Research-Routine work in great volume again prevent- ed much being done in the way of research.

(a) Dysentery.—The work on Flexner dysentery strains commenced during the latter part of 1932 has been pursued steadily and although the number of cultures isolated and dealt with is not large, some facts emerging are of considerable in- terest.

The appended table shows the distribution of the strains between the various sub-divisions of the group and those which are inagglutinable.

V

W

X

Y

Z

ZX

7%

28%

13%

7%

2%

28%

9%

6%

Mixed

Inagglutinable

The infrequence of inagglutinable strains is somewhat re- markable in view of the records of Indian observers. Thus Manifold and deMonte report 28.2% of 117 strains as inagglutin- able. It is possible that the reason for this may be in the fact that in the latter group agglutination was carried out relatively soon after isolation, whereas in our series all strains isolated were subcultured weekly for three months before agglutination, thus permitting any agglutinable property to become manifest and fixed. The type sera used were those of Medical Research Council and all the strains recorded showed agglutination to 50% or over titre. Boyd's experience regarding saccharose fer- mentation has been confirmed by us, no agglutinable culture showing true saccharose fermentation. One of the inagglutin- able cultures, however, showed definite acidity in saccharose. This strain has not yet been investigated thoroughly.

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