AnnualReport-1932 — Page 478

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M 133

done by him between 9.30 and 12.30 a.m. and the results of the tent catches of the previous night obtained. Additional morning catches were done from time to time by the other probationers as well. On reaching the Laboratory the mosquitoes were transferred to small lamp glasses both ends of which were covered with netting. These were placed in a box the bottom of which was lined with lint always kept moist. Raisins were placed on top of the lamp glasses for the mosquitoes to feed upon. The box is provided with legs which stand in saucers containing a disinfectant to prevent ants gaining access. The dissections were done by the aid of the binocular microscope in the usual manner. All positive results of dissections were submitted to the Malariologist before being recorded.

Results of catches and dissections are shown in Tables VII, VIII, IX, X, XI. In this neighbourhood the best places for obtaining Anophelines in the morning catches, were in the palm leaf huts around the village; they were not so readily found in the village houses built of stone and roofed with tiles. During one morning, two probationers spent the same time, one in searching huts, the other in searching village houses. Nineteen Anophelines were obtained from the huts, six from the houses. The Anophelines were most commonly met with on the dingy mosquito curtains, and some on the sides of the huts and beneath tables in dark corners. An uninhabited hut used as a tool shed was repeatedly searched but only on one occasion was an Anopheline found in it, an A. minimus, although they were frequently found in adjacent huts. No Anophelines were found in empty houses or in the tent if unoccupied the previous night. This would indicate that a blood meal and not shelter was the object of their frequenting habitations. Numerous pig sties were searched but harboured no Anophelines; these, however, have low walls and high roofs supported on pillars and would not be likely resting places. There were no cowsheds. A. minimus was the commonest Anopheline found in day and night catches; it was caught at all hours from dusk until dawn; in the last quarter of the year A. jeyporiensis was encountered in comparatively large numbers. In most larval surveys the larvae of A. maculatus are in great majority, especially when compared with A. minimus, but in these catches the adults were scarce and that in an area where their larvae were numerous at all times. Few A. hyrcanus were captured but their larvae are not found abundantly in hill streams. It is proposed to move the tent closer to a swamp where their larvae have been obtained in large numbers and which is near habitations from which infected Anophelines have been obtained. The first infection recorded was in April, one oocyst 15 x in diameter in an A. minimus, and the first sporozoites were met with also in an A. minimus in the middle of June. The inhabitants of the locality rendered every assistance and informed the investigators that malaria did not start until July, a statement which was confirmed in this instance by the results of the dissections of

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M 133 done by him between 9.30 and 12.30 a.m. and the results of the tent catches of the previous night obtained. Additional morning catches were done from time to time by the other probationers as well. On reaching the Laboratory the mosquitoes were transferred to small lamp glasses both ends of which were covered with netting. These were placed in a box the bottom of which was lined with lint always kept moist. Raisins were placed on top of the lamp glasses for the mosquitoes to feed upon. The box is provided with legs which stand in saucers containing a disinfectant to prevent ants gaining access. The dissections were done by the aid of the binocular microscope in the usual manner. All positive results of dissections were submitted to the Malariologist before being recorded. Results of catches and dissections are shown in Tables VII, VIII, IX, X, XI. In this neighbourhood the best places for obtaining Anophelines in the morning catches, were in the palm leaf huts around the village; they were not so readily found in the village houses built of stone and roofed with tiles. During one morning, two probationers spent the same time, one in searching huts, the other in searching village houses. Nineteen Anophelines were obtained from the huts, six from the houses. The Anophelines were most commonly met with on the dingy mosquito curtains, and some on the sides of the huts and beneath tables in dark corners. An uninhabited hut used as a tool shed was repeatedly searched but only on one occasion was an Anopheline found in it, an A. minimus, although they were frequently found in adjacent huts. No Anophelines were found in empty houses or in the tent if unoccupied the previous night. This would indicate that a blood meal and not shelter was the object of their frequenting habitations. Numerous pig sties were searched but harboured no Anophelines; these, however, have low walls and high roofs supported on pillars and would not be likely resting places. There were no cowsheds. A. minimus was the commonest Anopheline found in day and night catches; it was caught at all hours from dusk until dawn; in the last quarter of the year A. jeyporiensis was encountered in comparatively large numbers. In most larval surveys the larvae of A. maculatus are in great majority, especially when compared with A. minimus, but in these catches the adults were scarce and that in an area where their larvae were numerous at all times. Few A. hyrcanus were captured but their larvae are not found abundantly in hill streams. It is proposed to move the tent closer to a swamp where their larvae have been obtained in large numbers and which is near habitations from which infected Anophelines have been obtained. The first infection recorded was in April, one oocyst 15 x in diameter in an A. minimus, and the first sporozoites were met with also in an A. minimus in the middle of June. The inhabitants of the locality rendered every assistance and informed the investigators that malaria did not start until July, a statement which was confirmed in this instance by the results of the dissections of
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M 133 done by him between 9.30 and 12.30 a.m. and the results of the tent catches of the previous night obtained. Additional morning catches were done from time to time by the other probationers as well. On reaching the Laboratory the mosquitoes were trans- ferred to small lamp glasses both ends of which were covered with netting. These were placed in a box the bottom of which was lined with lint always kept moist. Raisins were plured on top of the lamp glasses for the mosquitoes to feed upon. The box is provided with legs which stand in saucers containing a disinfectant to prevent ants gaining access. The dissection were done by the aid of the binocular microscope in the usual manner, All positive results of dissections were submitted to the Malario- logist before being recorded. Results of catches and dissections are shown in Tables VII, VIII, IX, X, XI. In this neighbourhood the best places fɔr obtaining Anophelines in the morning catches, were in the palm leaf huts around the village; they were not so readily found in the village houses built of stone and roofed with by tiles. During one morning, two probationers spent the same time, one in searching huts, the other in searching village houses. Nineteen Anophelines were obtained from the huts, six from the houses. The Anophelines were most commonly met with on the dingy mosquito curtains, and some on the sides of the huts and beneath tables in dark corners. An uninhabited hut used as a tool shed was repeatedly searched but only on one occasion was an Ano- pheline found in it an A. minimus, although they were fre- quently round in adjacent huts. No Anophelines were found in empty houses or in the tent if unoccupied the previous night. This would indicate that a blood meal and not shelter was the object of their frequenting habitations. Numerous pig sties were searched but harboured no Anophelines; these, however, have low walls and high roofs supported on pillars and would not be likely resting places. There were no cowsheds. A. minimus was the commonest Anopheline found in day and night catches, it was caught at all hours from dusk until dawn; in the last quarter of the year A. jeyporiensis was encountered in compara- tively large numbers. In most larval surveys the larvae of A. maculatus are in great majority especially when compar- ed with A. minimus, but in these catches the adults were scarce and that in an area where their larvae were numerous at all times. Few A. hyrcanus were captured but their larvae are not found abundantly in hill streams. It is proposed to move the tent closer to a swamp where their larvae have been obtained in large numbers and which is near habitations from which infected Anophelines have been obtained. The first infection recorded was in April, one oocyst 15 x diameter in an A. minimus, and the first sporozoites were met with also in an A. minimus in the middle of June. The inhabitants of the locality rendered every assistance and informed the investigators that malaria did not start until July, a statement which was confirmed in this instance by the results of the dissections of
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M 133

done by him between 9.30 and 12.30 a.m. and the results of the tent catches of the previous night obtained. Additional morning catches were done from time to time by the other probationers as well. On reaching the Laboratory the mosquitoes were trans- ferred to small lamp glasses both ends of which were covered with netting. These were placed in a box the bottom of which was lined with lint always kept moist. Raisins were plured on top of the lamp glasses for the mosquitoes to feed upon. The box is provided with legs which stand in saucers containing a disinfectant to prevent ants gaining access. The dissection were done by the aid of the binocular microscope in the usual manner, All positive results of dissections were submitted to the Malario- logist before being recorded.

Results of catches and dissections are shown in Tables VII, VIII, IX, X, XI. In this neighbourhood the best places fɔr obtaining Anophelines in the morning catches, were in the palm leaf huts around the village; they were not so readily found in the village houses built of stone and roofed with by tiles. During one morning, two probationers spent the same time, one in searching huts, the other in searching village houses. Nineteen Anophelines were obtained from the huts, six from the houses. The Anophelines were most commonly met with on the dingy mosquito curtains, and some on the sides of the huts and beneath tables in dark corners. An uninhabited hut used as a tool shed was repeatedly searched but only on one occasion was an Ano- pheline found in it an A. minimus, although they were fre- quently round in adjacent huts. No Anophelines were found in empty houses or in the tent if unoccupied the previous night. This would indicate that a blood meal and not shelter was the object of their frequenting habitations. Numerous pig sties were searched but harboured no Anophelines; these, however, have low walls and high roofs supported on pillars and would not be likely resting places. There were no cowsheds. A. minimus was the commonest Anopheline found in day and night catches, it was caught at all hours from dusk until dawn; in the last quarter of the year A. jeyporiensis was encountered in compara- tively large numbers. In most larval surveys the larvae of A. maculatus are in great majority especially when compar- ed with A. minimus, but in these catches the adults were scarce and that in an area where their larvae were numerous at all times. Few A. hyrcanus were captured but their larvae are not found abundantly in hill streams. It is proposed to move the tent closer to a swamp where their larvae have been obtained in large numbers and which is near habitations from which infected Anophelines have been obtained. The first infection recorded was in April, one oocyst 15 x diameter in an A. minimus, and the first sporozoites were met with also in an A. minimus in the middle of June. The inhabitants of the locality rendered every assistance and informed the investigators that malaria did not start until July, a statement which was confirmed in this instance by the results of the dissections of

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