L 64
(4.) About 4-6 colonies showing Acid and Gas change from each plate are picked off on to Agar Slopes, incubated about 18 hours at 37° C. and then tested for morphology, Gram staining and motility.
Then from each Agar Slope the following tubes are put up:-(1) Gelatine (for liquefaction or not), (2) Peptone water (for Ehrlich's Indel Reaction), (3) Litmus milk (for Acid and Clot), (4) Lactose, (5) Glucose, (6) Mannite, (7) Saccharose, (8) Dulcite, (9) Adonit, (10) Inosit, (11) Inulin, all for Acid and Gas, (12) Neutral-Red Glucose Broth (for florescence).
The Glucose tube is also used for the Vosges and Proskawer's Reaction. The production of acidity in Litmus whey and the reduction of Nitrates may or may not also be tested for. From these reactions the characters of the Lactose Fermenters are determined and their relative numbers in the sample of water fixed.
Bacillus Enteritidis and Streptococci are not tested for as a routine matter as it does not appear to be worth the trouble, and a sample which does not contain B. Coli or Coli-like organisms in 50 cc. is not investigated further. No opinion is given as to Pathogenic organism, the report being on the total number of organisms per cc. on Agar at 37° C. and the relative number and position of B. Coli or Coli-like organisms.
The method of preparing the media and of classifying the Lactose Fermenters closely follows that described by MacConkey (Journal of Hygiene Vol. 8 p. 322 and Vol. 9 p. 86).
MATERIALS SENT FOR EXAMINATION AND REPORT.
These amounted to 406 compared with 120 for the year 1909. Table III briefly gives the nature of the examination made.
New Growths-Examination by Section, 33 Widal Reaction for Typhoid, 106 ,, Paratyphoid, 17 ,, Dysentery, 2 Cultural Examination for Typhoid Carriers, 4 Culture for Diphtheria Bacillus, 99 Microscopical Examination for B. Tubercle, 11 Animal Inoculation for Tubercle, 6 Examination of Dejecta for Cholera, 24 Blood for Widal Reaction against Cholera Vibrios, 6 Examination of Material for Gonococcus, 2 Pneumococcus, 2 Glanders, 1 Plague, 3 Micrococcus Intracellularies, 1 Mycetoma, 1 Carried forward. 309L 64
w
(4.) About 4-6 colonies showing Acid and Gas change from each plate are picked off on to Agar Slopes, incubated about 18 hours at 37° C. and then tested for morphology, Gram staining and motility.
Then from each Agar Slope the following tubes are put up :-(1) Gelatine (for liquefaction or not), (2) Peptone water (for Ehrlich's Indel Reaction), (3) Litmus milk (for Acid and Clot), (4) Lactose, (5) Glucose, (6) Mannite, (7) Saccharose, (8) Dulcite, (9) Adonit, (10) Inosit, (11) Inulin, all for Acid and Gas, (12) Neutral-Red Glucose Broth (for florescence).
The Glucose tube is also used for the Vosges and Proskawer's Reaction. The production of acidity in Litmus whey and the re- duction of Nitrates may or may not also be tested for. From these reactions the characters of the Lactose Fermenters are determined and their relative numbers in the sample of water fixed.
Bacillus Enteritidis and Streptococci are not tested for as a routine matter as it does not appear to be worth the trouble, and a sample which does not contain B. Coli or Coli-like organisms in 50 cc. is not investigated further. No opinion is given as to Pathogenic organism, the report being on the total number of organisms per cc. on Agar at 37° C. and the relative number and position of B. Coli or Coli-like organisms.
The method of preparing the media and of classifying the Lactose Fermenters closely follows that described by MacConkey (Journal of Hygiene Vol. 8 p. 322 and Vol. 9 p. 86).
MATERIALS SENT FOR EXAMINATION AND REPORT.
These amounted to 406 compared with 120 for the year 1909. Table III briefly gives the nature of the examination made.
Table III.
New Growths-Examination by Section,
Widal Reaction for Typhoid,
33
106
8888888
>
17
,,
Paratyphoid,
Dysentery,
2
Cultural Examination for Typhoid Carriers,.
4
Culture for Diphtheria Bacillus,.
99
Microscopical Examination for B. Tubercle,
11
Animal Inoculation for Tubercle,
6
Examination of Dejecta for Cholera,..........
24
Blood for Widal Reaction against Cholera Vibrios, Examination of Material for Gonococcus,
6
2
}}
Pneumococcus,
2
Glanders,
1
>
>>
>>
>>
Plague,
3
>>
Micrococcus Intracellularies,
1
Mycetoma,
1
Carried forward.
309
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