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16th December.-Temperature, 103° F., but blood examination gave a negative result.

21st December.-Temperature, 103 6o, but blood still showed absence of try- panosomes.

Finally, on 30th December the blood was again examined, but with a negative result. The dog at present is in good condition and seems to be in good health; the temperature, although not running a regular course, does not rise above the normal maximum. The animal is still being kept under observation.

Calf A.-Poor in condition. (See Charts Nos. 13 and 14.) 27th September.--Blood examined, no trypanosomes found.

Inoculated with 5 c.c. of blood from dog A. Afternoon temperature steadily

rose daily.

3rd October.-Temperature, 105° F., trypanosomes present in the blood. 6th October.-Treatment begun.

15th October.-Treatment ended.

16th October.-Temperature, 103° F. Blood examined, but no trypanosomes found.

22nd October.-Blood examined, but result negative.

25th October.-Temperature, 105° F., but blood examination gave a negative result.

After the 22nd the chart will show the great fluctuations in the morning and evening temperature, which continued until death.

4th November.-The. calf died, and the blood, on examination immediately after death, showed no trypanosomes.

On the 29th October dog D was inoculated with 5 c.c. of blood from this calf, when the blood, after three examinations, gave negative results, but the tempera- ture continued high. This dog, as will be seen from the next case described, developed the disease and died."

Thus it may be concluded that either (a) the treatment caused the organisms to pass into phase B of its life-cycle, or (b) the organisms existed in the peripheral circulation, but owing to their paucity were not detected in the three blood examinations made during the life of the animal. If, however, the former was the case, then it is likely that phase B of the life cycle may not only occur in some unknown internal organ, but also in the peripheral blood stream, either primarily or secondarily, by being liberated into the blood stream from the organs where it survives in the form of phase B. It is, however, most probable that, owing to the fewness of the organisms in the blood stream, examinations failed to detect them.

Dog D. (See Charts 15 and 16.)

29th October.-Blood examined and no trypanosomes found.

Inoculated with 5 c.c. of blood from calf A.

10th November-Temperature, 106 2° F., and examination of blood showed

the presence of trypanosomes.

On 15th and 17th November trypanosomes were found present in the blood. On 22nd November the dog died.

No treatment was administered to this dog.

Calf B.-In good condition. (See Charts Nos. 17 and 18.)

1st November.-Blood examined and no trypanosomes found.

8th November.-Blood examination gave negative result.

Inoculated with 5 c.c. blood from horse B.

18th November.-A great many trypanosomes found in the blood.

19th November.-Treatment begun.

23rd November.-Blood examined, result positive.

28th November-Tenth day of treatment.

30th November-Two days after cessation of treatment the examination of

the blood gave a negative result, and at this stage 8 c.c. of blood were inoculated into calf D, which subsequently developed the disease.

5th December. Another blood examination gave a negative result.

10th December.-Blood examination again showed the presence of trypano-

somes.

30th December. The blood again gave a positive result.

During the last three weeks the calf fell off very considerably in condition and broke out with a papular eruptía, which scaled off, leaving a surface denuded of hair. This result was also seen in case of calf A.

On 1st January, 1908, calf B died.

Remark. In this case it will also be seen that at a time when the blood, on examination, showed absence of trypanosomes, yet the inoculation of 8 c.c. blood reproduced the disease.

Calf E-In good condition. (See Charts Nos. 19A and 198.)

30th November.-Blood examination showed no trypanosomes.

Inoculated with 8 c.c. blood from a black Government ox suffering naturally from the disease.

7th December.-Temperature, 104-4° F. Blood examinations showed the pre sence of trypanosomes.

12th December.-Blood examination, result positive. Treatment begun. 21st December.-Tenth and last day of treatment.

30th December. The blood was examined, but trypanosomes were still present

and the temperature continues to run high. The animal is falling off in condition greatly, and the future outlook is unfavourable.

Black Government Ox No. 1.—In good condition, but when seen by me quite unable to rise. A case of natural infection.

28th October.-Blood examination, and trypanosomes found.

Treatment begun and 18 c.c. of the mixture injected into jugular vein.

1st November.-The animal died 5 days after treatment was begun. No chart

was kept of this case.

Black Government Ox No. 2.-(See Chart No. 21.)

This was also a case of natural infection. It was brought for treatment when found incapable of work; it could, however, stand and walk.

22nd November-Blood examined, result positive.

25th November.-Blood examined, result positive. Treatment begun. 30th November.-Blood examined, but no trypanosomes seen.

At this stage

8 c.c. of blood were inoculated into calf E, which developed the disease (see page 11).

4th December.-Tenth and last day of treatment.

5th December.-Blood examination gave a positive result.

After the 1st December the daily fluctuations in temperature were extreme. The animal died on the 9th December.

Remark. In this case also, when the blood gave a negative result on examina-

tion and 8 c.c. were inoculated info calf E, the disease was reproduced.

10. Brief Summary of Results:—

Two Dogs, A and B.-In both these treatment was attempted subcutaneously, but it proved a failure. Both dogs died.

In two Horses-Both infected by inoculation. In horse A, poor in condition, result of treatment was a failure. Animal succumbed.

In horse B, in good condition, treatment resulted in partial success. remarks on the case, pages 6 to 9.) Animal under observation and experiment.

Three Calves.-All infected by inoculation. Calves A, B, and E.

(See

Treatment

a failure in all three. Two already died; one living, but condition not hopeful.

Two Oxen, Nos. 1 and 2.--Both cases of natural infection.

In case of No. 1, the disease was much advanced when the case was taken in The animal died on the sixth hand, and the result of treatment was unsuccessful.

day of treatment.

In the case of No. 2, the disease was not so far advanced as in No. 1, but the treatment proved a failure, and the animal died after the treatment.

11. I regret that I have not been able to test the treatment more fully on horses, as their cost is prohibitive. Further experiments should be conducted on horses as I have already said under my remarks on pages 8 and 9.

12. In conclusion, I wish to say that I am greatly indebted to Major McClintock, the Resident, for the invaluable aid he has afforded me by providing me with most of the animals experimented on, and the cost of maintaining them to a great extent, as well as helping me in many other respects in connection with the work, for which I wish to take this opportunity of expressing my thanks to him I have also to thank Mr. Elder, the Assistant Resident, for the help he has also given me in furthering the work.

H. A. Fox,

Maiduguri,

January 1, 1908.

Medical Officer.

this case it will also be seen that at a time when the blood, on wed absence of trypanosomes, yet the inoculation of 8 c.c. blood isease.

good condition. (See Charts Nos. 19A and 198.)

ber. Blood examination showed no trypanosomes.

with 8 c.c. blood from a black Government ox suffering naturally

r.-Temperature, 104-4° F. Blood examinations showed the pre

lomes.

>er-Blood examination, result positive. Treatment begun. er.-Tenth and last day of treatment.

er. The blood was examined, but trypanosomes were still present cure continues to run high. The animal is falling off in condition future outlook is unfavourable.

nment Ox No. 1.-In good condition, but when seen by me quite A case of natural infection.

r. Blood examination, and trypanosomes found.

egun and 18 c.c. of the mixture injected into jugular vein.

r-The animal died 5 days after treatment was begun. No chart

case.

rment Ox No. 2.-(See Chart No. 21.)

o a case of natural infection. It was brought for treatment when

of work; it could, however, stand and walk.

ber-Blood examined, result positive.

ber. Blood examined, result positive. Treatment begun.

ber. Blood examined, but no trypanosomes seen.

At this stage

were inoculated into calf E, which developed the disease (see

er. Tenth and last day of treatment.

er.--Blood examination gave a positive result.

st December the daily fluctuations in temperature were extreme.

on the 9th December.

a this case also, when the blood gave a negative result on examina- ere inoculated info calf E, the disease was reproduced.

Summary of Results:-

A and B.-In both these treatment was attempted subcutaneously, failure. Both dogs died.

ses-Both infected by inoculation. In horse A, poor in condition, ent was a failure. Animal succumbed.

in good condition, treatment resulted in partial success. (See case, pages 6 to 9.) Animal under observation and experiment.

Treatment 's. All infected by inoculation. Calves A, B, and E.

three. Two already died; one living, but condition not hopeful. Nos. 1 and 2.--Both cases of natural infection.

No. 1, the disease was much advanced when the case was taken in esult of treatment was unsuccessful. The animal died on the sixth it.

of No. 2, the disease was not so far advanced as in No. 1, but the

d a failure, and the animal died after the treatment.

et that I have not been able to test the treatment more fully on cost is prohibitive. Further experiments should be conducted on already said under my remarks on pages 8 and 9.

nclusion. I wish to say that I am greatly indebted to Major > Resident, for the invaluable aid he has afforded me by providing f the animals experimented on, and the cost of maintaining them it, as well as helping me in many other respects in connection with hich I wish to take this opportunity of expressing my thanks to him. thank Mr. Elder, the Assistant Resident, for the help he has also thering the work.

H. A. Fox,

Medical Officer.

1908.

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